1. 32 amino acids is a critical length
rDNA origin
synthetic
recombinant
The mean bioavailability of Calcitonin-Salmon Nasal Spray is
approximately 3% of that of injectable calcitonin in normal subjects.

2. Human calcitonin : Not N-glycosylated modified C-terminus !
1 disulfide
bridge
Not N-glycosylated 32
prolineamide
modified C-terminus !

3. Why is salmon calcitonin more potent than human calcitonin ?
The conformation hypothesis seems the most likely .

4. Calcitonin : salmon human Identity : 16 aa / 32 aa Ser Asn Thr Val Lys
Leu
Ser
Glu
Leu
His
Leu
Gln
Tyr
Arg
Ser
Identity : 16 aa / 32 aa
Thr

5. Comparison human / salmon calcitonin
(amino acids sequence)
salmon:
Cys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-
Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Gly-
Thr-Pro
human:
Cys-Gly-Asn-Leu-Ser-Thr-Cys-Met-Leu-Gly-Thr-Tyr-Thr-Gln-Asp
-Phe-Asn-Lys-Phe-His-Thr-Phe-Pro-Gln-Thr-Ala-Ile-Gly-Val-Gly-
Ala-Pro

6. Osteoporosis 50% bone mass loss after menopausis
Young 2013 EPFL student
50% bone mass loss
after menopausis

8. nomal
osteopenia
osteoporosis

9. Osteoporosis decreased bone density 1 5 Lumbar vertebrae : the last 5
Hoefnagel

10. osteoporosis
L2 : 2nd lumbar vertebra

11. Vertebrae of 40- vs. 92-year-old women
Note the marked loss of trabeculae with preservation of cortex.

12. 2 problems linked to osteoporosis :
- vertebral compression fracture
- hip fracture

13. Densitometry
measuring bone density

14. Bilateral ovaryectomy (OVX) in goats  osteoporosis
Large animal
models can be
investigated
using the same
instruments as
for humans.
BMD : Bone Mass Density
OVX induces bone mass loss confirming the link between
menoposis and osteoporosis

15. CALCITONIN prevention no effect 2 major risks of osteoporosis :
vertebral fracture
hip fracture

16. Administration routes for recombinant proteins.
injecteur
projecteur
(sans aiguille)
nasal spray

18. Oxytocyn : CYIQNCPLG 9 amino acids modified C-terminus !
Nasalspray 1 Sprühstoss (0.1 ml Nasalspray Lösung zu 4 I.E. Oxytocin) in eine der beiden Nasenöffnungen 5 min vor dem Stillen oder Abpumpen der Milch.
modified C-terminus !

19. Syntocinon nasal spray :
medical use : lactation help
published in 2009

20. These 4 administration routes are nonsense
Human growth hormone : 191 aa.

22. Progress in DNA synthesis :

23. Chemical DNA synthesis:
Synthétiseur automatique d’ADN: