1. Microscope Lab
Do Now: Pick up lab and preread the whole lab, answer pre-lab questions when finished reading. Not before, after!

2. Set up your Cornell Notes As Follows (skip lines……):
I. Safety
II. Magnification
III. Preparing Wet Mount
IV. Focusing
V. Electron Microscopes
Define: resolving power, depth of view

3. Compound Light Microscope I. Safety
Always carry with 2 hands
Arm and base
Place away from edge of table
Always start with
stage all the way down
Light off
Lowest power
Always return the microscope to this position when finished

6. Eyepiece Course Focus Fine Focus Scanning Obj Arm Power Body Tube
Nose piece
High Power
Low Power
Clips
Stage
Diaphragm
Light
Base 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

7. II. Magnification Eyepiece Rotating nosepiece has 3 objectives
What you look through
May have pointer inside
10x
Rotating nosepiece has 3 objectives
Scanning (short and red) 4x
Low (medium and yellow) 10x
High (long and blue) 40 x

8. Total Magnification = Eyepiece X objective
Scanning 4x = 40x total
Low 10x = 100x total
High 40x = 400x total
Eyepiece
10x

9. Controlling light First plug in and turn on the light.
Under the stage is a rotating disc that turns and clicks
It may have numbers on it
Turning the diaphragm (disc) controls the amount of light

11. Slide #1- hair Slide #2 - letter “e”
Prepared slide of crossed threads

12. III. Preparing Wet Mount
1. Place letter e in the middle of your slide.
2. place 3 drops of water on top of your letter e
3. put cover slip in the edge of the puddle of water and release
4. do not press or move the cover slip

13. Place slide on stage and secure
IV. Focusing
Place slide on stage and secure
Use scanning objective and try to center the crossed threads then focus
Use course focus (large knob) first
Fine focus (small knob) second
Which way does the slide move if you push it left? Push right?
What affect does changing the diaphragm have on the image?
Change to low power
Notice any change?
Draw

14. Change to high power USE ONLY FINE FOCUS!
Course focus can press into slide and crack the glass, squish specimen, and damage the objective.
It may take time to focus correctly - practice
How does the view of the image change?
Draw
don’t SQUISH THE SLIDE!!!!!! 

15. The Wonderful Letter e Preparing a wet-mount
Cut out a lower case letter e
Place on slide
Carefully add water drop
Try to keep the e looking like this… e
Carefully place cover slip on letter

16. Place slide on microscope and secure
Start with scanning objective - center and focus. See any thing unusual?
Change to low power and draw
Change to high power and draw

17. Depth of View
Notice that as you increase magnification you see more detail of less of the image.
You also see layer of the image that you can focus through
More of less

18. Resolving Power
A measure of the clarity of an image.

19. Closure
Complete post lab questions
Turn in lab

20. V. Electron Microscopes
Scanning or SEM
Uses electrons bounced off the surface of the specimen
Transmission or TEM
Electrons through the specimen
Live specimens cannot be used

21. Electron Microscope (2 kinds)*

22. a)Transmission Electron Microscope
TEM- Electrons go Through the Specimen
(non living SpecimensOnly)

23. b)Scanning Electron Microscope
SEM –Uses electrons Bounced off the Surface of the Specimen
(non living Specimens only)