1. Types of Microscopes
Unit 4 Biology

2. Simple Microscopes Have only 1 lens
Give two examples of a simple microscope:
Magnifying Glass
Reading Glasses

3. Compound Microscopes
Have more than one ______
lens

4. Dissecting Microscopes
20x x
Magnify by 10x or ______ or ________
Shine light on or ____________ the specimen
Specimen can be ________ or thin.
through
thick

5. Dissecting Microscope

6. Light Microscopes
Use a beam of Light
The specimen can be dead or alive

7. 40- 400x through Light Microscope thin Magnify by ______________
Shines light ______________ the specimen
Specimen must be _________.
through
thin

8. Light Microscope Image

9. electrons dead air water Electron Microscope
Use a beam of _______________-
The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________
dead
air
water

10. 60,000x Scanning E.M. Makes 3D pictures of surfaces
Magnify by _________
60,000x

11. Scanning E.M. Image (3D)

12. Makes _____ pictures of thin slices
Transmission E.M. 2D
Makes _____ pictures of thin slices
Magnify by __________
100,000x

13. Transmission E.M. Image (2D)
Butterfly Antenna

14. _____________ can be seen with these 2 types of microscopes. Atoms
Other Microscopes
Probe Microscopes
Ion Microscopes
_____________ can be seen with these 2 types of microscopes.
Atoms

15. Probe Microscope

16. Probe Microscope Image

17. Ion Microscopes

18. Ion Microscope Images

19. Microscope Parts and Functions

20. 1. Eyepiece
Magnifies by 10x

21. Changes Objective Lens
2. Revolving Nose Piece
Changes Objective Lens

22. 3. Low power Objective Lens
Magnifies by 4x
(Red)

23. 4. Medium Power Objective Lens
Magnifies by 10x (yellow)

24. 5. High Power Objective Lens
Magnifies by 40x (Blue)

25. 6. Stage Clips
To hold slide in place

26. Adjusts the amount of light
7. Diaphragm
Adjusts the amount of light

27. 8. Light
Light to see specimen

28. 9. On/Off Switch
Turns light on and off

29. 10. Arm
To hold while carrying

30. 11. Stage
To set slide on

31. To focus only when in Low Power
12. Course Adjustment
To focus only when in Low Power

32. To focus when using medium or high power
13. Fine Adjustment
To focus when using medium or high power

33. 14. Base
To hold while carrying

34. Both Arm Base Eyepiece Away Teacher Lens Tissue
A. Carry a microscope with ___________ hands – one hand holding the _______
And the other hand under the ___________
Always hold the microscope upright so the _____________ cannot fall out
When you place a microscope at your table, make sure it is _______ from the edge
If there is anything wrong with the microscope, or it is damaged in any way, let the
_______________ know immediately.
B. To clean the objective lenses and eyepiece, use _______________________
Base
Eyepiece
Away
Teacher
Lens Tissue

35. Light Low 4X Slide Lens Highest Slide Coarse Focus Fine Diaphragm
Prepare your slide
Plug the microscope in. Turn on the ______________
Be sure the ___________ Power Objective Lens (_______) is clicked into place.
NOTE: Be sure to look at the microscope from the side when changing objective
lenses to avoid damaging the ________________ and _____________
4. Use coarse focus to raise the stage to its ________________ position
5. Place the _______________ on the stage.
Look through the eyepiece and turn the __________________ (lowering the stage)
until the specimen is visible.
7. Use the ____________ adjustment to bring your specimen into sharp focus.
You may wish to move the ________________ to adjust the amount of light.
**The Eyepiece has a black arrow on it for pointing out parts of a specimen
Light
Low 4X
Slide
Lens
Highest
Slide
Coarse Focus
Fine
Diaphragm

36. Focused Mid 10X Side Coarse Fine Focused Side High 40X Fine Low Cord
8. Make sure the specimen is ______________
9. Switch to the ___________ Power Objective Lens ( ______)
NOTE: Look at the microscope from the ____________ when you change lenses.
10. To avoid losing your focus and/or crushing the slide with the lens, never use the
__________________ focus on medium or high power.
Use the _____________ focus only to make the specimen visible.
11. Make sure the specimen is ______________. Look from the _________ as you
change objective lenses and switch to the __________ Power Lens (________)
12. Use only the _____________ focus to make the specimen visible.
13. When finished, make sure the ___________ power objective lens is in place,
wrap the __________ around the _________ of the microscope, cover, and place
in the appropriate location
Mid
10X
Side
Coarse
Fine
Focused
Side
High
40X
Fine
Low
Cord
Arm