1. Polyherbal Formulations for Diabetes
Prof. Dr. Basavaraj K. Nanjwade. M.Pharm., Ph.D.
Department of Pharmaceutics
KLE University College of Pharmacy
Belgaum, Karnataka , India
Cell No:

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Graphical Abstract
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Introduction
Plant formulation and combined extracts of plants are used a drug of choice rather than individual. Various herbal formulations such as diamed, coagent db, Diasulin, and hyponidd, are well known for their antidiabetic effects.
Polyherbal formulation of Annona squamosa and Nigella sativa is composed of medicinal plants (Table 1), which are traditionally used for antidiabetic and antihyperlipidemic activity. The present investigation was undertaken to study the effect of the polyherbal formulation of Annona sqamosa and Nigella sativa on lipidperoxidation and tissue lipid profile in streptozotocin induced diabetic rats.
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Research Background
Polyherbal formulation of Annona squamosa and Nigella sativa on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in streptozotocin induced diabetic rats. Aqueous extract of Polyherbal formulation of Annona squamosa and Nigella sativa was administered orally (200 mg/kg body weight) for 30 days.
The different doses of Polyherbal formulation on blood glucose and plasma insulin in diabetic rats were studied and the levels of lipid peroxides and tissue lipids were also estimated in streptozotocin induced diabetic rats. The effects were compared with tolbutamide. Treatment with Polyherbal formulation and tolbutamide resulted in a significant reduction of blood glucose and increase in plasma insulin.
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5. Annona squamosa (Sugar–Apple)
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Nigella sativa
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Material and methods
Animals
Preparation of drug
Chemicals
Drug administration
Streptozotocin-induced diabetes
Experimental design
Biochemical analysis
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Experimental design
In the experiment, a total of 42 rats (30 diabetic surviving rats, 12 normal rats) were used.
The rats were divided into seven groups of six rats each after the induction of streptozotocin diabetes.
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Experimental design
Group1: Normal treated rats
Group 2:Normal rats given aqueous solution of Polyherbal formulation (200 mg/kg body weight) daily using an intragastric tube for 30 days.
Group 3:Diabetic control rats.
Group 4: Diabetic rats given aqueous solution of Polyherbal formulation (50 mg/kg body weight) daily using an intragastric tube for 30 days.
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Experimental design
Group 5: Diabetic rats given aqueous solution of Polyherbal formulation (100 mg/kg body weight) daily using an intragastric tube for 30 days.
Group 6: Diabetic rats given aqueous solution of Polyherbal formulation (200 mg/kg body weight) daily using an intragastric tube for 30 days.
Group 7: Diabetic rats given aqueous solution of Tolbutamide (250 mg/kg body weight) daily using an intragastric tube for 30 days.
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Biochemical analysis
Estimation of blood glucose and plasma insulin
Estimation of lipid peroxidation
Estimation of lipids
i. Lipids
ii. For total cholesterol estimation
iii. Fro triglycerides estimation
iv. Phopholipids content
v. Free fatty acids
vi. Statistical analysis
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Table 1: Polyherbal Formulation of Annona Squamosa and Nigella sativa (Composition and Concentration)
Sl. No
Botanical Name
Common Name
Family
Part used
Conc. 1.
Annona squamosa
Sharifa
Annonnacceae
Matured fruits 50 2.
Nigella sative
Kalonji
Ranunculaceae
seeds
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13. Fasting blood glucose (mg/dI) Plasma insulin (IU/ml)
Table 2: Changes in blood glucose and plasma insulin levels of control and experimental animals
Group
Fasting blood glucose (mg/dI)
Plasma insulin (IU/ml)
Normal
81.04 ± 2.29
11.26 ± 0.96
Diabetic control
± 22.23
3.48 ± 0.69
Diabetic + Polyherbal formulation (50 mg/kg)
± 12.46
5.59 ± 0.34
Diabetic + Polyherbal formulation (100 mg/kg)
± 11.69
6.03 ± 0.45
Diabetic + Polyherbal formulation (200 mg/kg)
± 6.56
7.15 ± 0.45
Diabetic + Tolbutamide
(250 mg/kg)
± 9.35
6.32 ± 0.48
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Table 3: Changes in levels of TBARS and Hydroperoxides in liver and kidney of control and experimental animals
Groups
TBARS
Hydroperoxide
Liver
Kidney
Normal
0.58 ± 0.066
0.68 ± 0.05
70.38 ± 4.54
55.34 ± 4.55
Diabetic + Polyherbal formulation (200 mg/kg)
0.56 ± 0.079
0.78 ± 0.06
68.59 ± 5.14
52.48 ± 4.75
Diabetic control
1.54 ± 0.06
1.65 ± 0.12
99.98 ± 5.98
78.29 ± 4.58
0.64 ± 0.053
0.97 ± 0.07
80.55 ± 5.69
60.99 ± 4.78
Diabetic + Tolbutamide
(250 mg/kg)
0.67 ± 0.088
1.02 ± 0.08
84.35 ± 5.45
62.45 ± 5.56
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Table 4: Changes in levels of cholestrol, free fatty acids, triglycerides and phospholipids in liver of control and experimental animals ( mg/100g wet. tissue)
Groups
Cholesterol
Free fatty acids
Triglycerides
Phospholipids
Normal
± 18.90
± 1.76
± 23.20
± 19.30
Normal + Polyherbal formulation
(200 mg/kg)
± 5.74
± 9.00
± 21.00
± 24.10
Diabetic control
± 13.10
± 44.60
± 18.80
± 18.70
Diabetic + Polyherbal formulation
(200mg/kg)
± 17.54
± 3.30
± 17.30
± 14.40
Diabetic + Tolbutamide
(250 mg/kg)
± 9.79
± 3.77
± 35.70
± 17.60
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Table 5: Changes in levels of cholestrol, free fatty acids, triglycerides and phospholipids in kidney of control and experimental animals ( mg/100g wet. tissue)
Groups
Cholesterol
Free fatty acids
Triglycerides
Phospholopids
Normal + Polyherbal formulation
(200 mg/kg)
372.08±8.28
432.51±1.60
278.75±14.60
±43.30
Diabetic control
546.90±23.80
743.00±5.70
501.10±34.10
±33.60
Diabetic+Polyherbal formulation (200 mg/kg)
435.20±12.18
556.80±8.50
382.90±9.28
±28.80
Diabetic+ Tolbutamide
(250 mg/kg)
449.90±13.49
600.30±3.40
438.66±39.30
±34.70
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Conclusion
Polyherbal formulation of Annona squamosa and Nigella sativa, exert a significant antihyperlipidemic. This could be due to combined effect of Annona squamosa and Nigella sativa. Hence the antihyperlipidemic effect of polyherbal formulation of Annona squamosa and Nigella sativa in particular could be considered as of possible therapeutic value
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Liposomes
Spherical vesicles with a phospholipid bilayer
Hydrophilic
Hydrophobic
Hydrophobic
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Liposome Preparation
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Liposome Preparation
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Liposome Preparation
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Lipid Peroxidation
Most phospholipid liposomes contain unsaturated acyl chains as part of their molecular structure and susceptible to oxidative degradation. It can be minimized by the use of animal derived lipids like egg PC, which has less saturated lipids, use of light resistant containers, use of antioxidants are useful in minimizing oxidation.
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24. Diabetic Current Research
Mangifera indica(stem,fruits,etc) – Anacardiaceae – Mango
Gossypiumherbaceum(flowers,etc ) – Malvaceae – Cotton
Cocos nucifera(roots,etc) – Arecaceae – Coconut
Lawsonia inermis(bark,etc) – Lythraceae - Mendhi
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My Research Group
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26. THANK YOU E-mail: bknanjwade@yahoo.co.in Cell No: 0091 9742431000
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