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Greatest Amplification:
Legend: Antigen Biotin Antigen Avidin Step 1 Primary Antibody (with light and heavy chains) (use BSA) Enzyme Step 2 Biotinylated Secondary Antibody (use BSA) Avidin (Neutravidin, Extravidin, Streptavidin) with Step 3 (use TTBS) Enzyme (HRP - horseradish peroxidase ) Step 4 ECL Light Lesser Amplification: Antigen Antigen Primary Antibody (with light and heavy chains) (use BSA) Primary Antibody (with biotin) (use BSA) Step 1 Step 1 Avidin with Secondary Antibody with HRP **do not use BSA (TTBS) Step 2 (use TTBS) Step 2 HRP Step 3 Step 3 ECL Light ECL Light
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