1. Introduction to Microscopy
VIBS 443/602

2. Use your atlas! Use your atlas! Use your atlas!
pancreas
blood
stomach
Atlas of Normal Histology by DiFiore
testis

3. Getting Started in the Laboratory
Light microscope parts
page 24
Kohler illumination pages 34-35

4. Kohler illumination (order is important)
Focus specimen
Close field diaphragm
Focus condenser
Center condenser
Open field diaphragm

5. Kohler illumination Focus specimen Close field diaphragm
Focus condenser
Center condenser
Open field diaphragm

6. MAGNIFICATION VS. RESOLUTION
MAGNIFICATION - INCREASE IN IMAGE SIZE
2. RESOLUTION - SMALLEST DISTANCE BETWEEN TWO POINTS THAT CAN BE SEEN (DISTINGUISHED)
CALCULATED BY
0.61 (WAVELENGTH)/NUMERICAL APERTURE
0.25 um FOR LIGHT MICROSCOPE
0.1 nm FOR ELECTRON MICROSCOPE

9. Peripheral blood smear (May-Grunwald-Giemsa)
113
Peripheral blood smear (May-Grunwald-Giemsa)

10. Peripheral blood smear (Leishman-Giemsa)
110
Peripheral blood smear (Leishman-Giemsa)

11. Peripheral blood smear (Leishman-Giemsa)
110
Peripheral blood smear (Leishman-Giemsa)

12. Peripheral blood smear (Leishman-Giemsa)
110
Peripheral blood smear (Leishman-Giemsa)

13. Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils
110

14. Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils
110

16. Pancreas
158

17. Pancreas
158

18. Pancreas, monkey (toluidine blue)
156
Pancreas, monkey (toluidine blue)

19. Pancreas, monkey (toluidine blue)
156
Pancreas, monkey (toluidine blue)

21. 165
UT165 larger secondary spermatocytes and smaller Golgi phase spermatids human testis

22. Testis - blood and lymph vessels
19680
Testis - blood and lymph vessels

23. 19709 Transparency of unstained tissue

24. 19709 Transparency of unstained tissue

25. Mast cell in unstained tissue
19709
Mast cell in unstained tissue

26. Mast cells in stained testicular tissue
19709

27. Mast cells in stained testicular tissue
19709
Mast cells in stained testicular tissue

29. 145
Fundic stomach

30. 145
Fundic stomach

31. Argentaffin cells of Fundic stomach, rabbit (toluidine blue)
244
Argentaffin cells of Fundic stomach, rabbit (toluidine blue)

32. Mucus neck cells Fundic stomach, rabbit (toluidine blue)
244
Mucus neck cells Fundic stomach, rabbit (toluidine blue)

33. EM 8f
EM 12a
EM 4c
EM 6a
Compare sizes of
membranes
ribosomes
mitochondria
EM 2b
EM 7

34. EM 8f: Peripheral blood cells; 9,000x
Monocyte
Lymphocyte
Neutrophil

35. EM 12a: Bone marrow; 13,200x. Note the reticular cell and developing red blood cells.

36. EM 4c: Intestinal absorption cell; 60,000x
Budding RER
Coated vesicle
Golgi
Mitochondria
Nucleus
Plasma membrane
Primary lysosome

37. EM 2b: Liver; 60,000x; cytoskeletal elements. Microtubes,
microfilaments, and intermediate filaments can be compared in this
cell, which has a high concentration of cortical microfilaments.
Microtube
Microfilaments
Intermediate filaments

38. EM 7: Ascites fluid; 80,000x. Clear examples of Golgi apparatuses with their cisternae and vesicles are present in this cell
Golgi apparatus
Ribosomes
Lipofuscin
Mitochondrion

39. EM 6a: Centriole-microtubules; 200,000x
EM 6a: Centriole-microtubules; 200,000x. Centriolar region of a cell showing both the stable, triplet microtubule arrays within the centriole, and the labile, individual microtubules originating from pericentriolar material.
Centriole
Stable microtubule
Labile microtubule

40. EM 4c 60,000x
EM 8f 9,000x
Compare sizes of
membranes
ribosomes
mitochondria
EM 12a 13,200x

41. EM 6a 200,000x
EM 2b 60,000x
Compare sizes of
membranes
ribosomes
mitochondria
EM 7 80,000x

42. Conventional TEM,
SEM
carbon replica TEM
EM 2b
EM 4a
EM 18b
EM 6
EM 7
EM 8

47. Conventional TEM,
SEM
carbon replica TEM
EM 2b
EM 4a
EM 18b
EM 6
EM 7
EM 8

48. Conventional TEM,
SEM
carbon replica TEM
EM 2b
EM 4a
EM 18b
EM 6
EM 7
EM 8

49. Conventional TEM,
SEM
carbon replica TEM
EM 2b
EM 4a
EM 18b
EM 6
EM 7
EM 8

50. Use your atlas! Use your atlas! pancreas blood stomach
Atlas of Normal Histology by DiFiore
testis