1. September 9, 2016 Objectives: Agenda:
Students will be able to use entomological patterns and weather data found in a case study to determine Time of Death (TOD).
Agenda:
Turn in homework
Warm Up: What in a crime scene can affect entomology data?
Observing the information provided by a climatological data sheet.
Determine TOD of a case study - together
Calculating accumulated degree hours (ADH) of the life stages of a fly
Calculating ADH from climatological data
Comparing the calculations to determine TOD of the victim
Independent assignment

2. Think-Pair-Share Background: All they could find was 1st instar
Found a family of three extremely decomposed bodies. Medical examiners predicted the bodies to be dead for months but could only find 1st instar stage flies. They need pupa flies to confirm this prediction. Why?
______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________
Background: All they could find was 1st instar

3. Case Study Bodies discovered at 1:00PM on June 20
Insects collected at 3:00PM on June 20
Victims had the same species and lifecycles present (2nd Instar)
Collection day hours: (June 20th)
Amount of am + amount of pm = hours
- _____________________

4. DEPAR-TURE FROM NORMAL HEATING COOLING TOTAL WATER EQUIV
DAY
MAX
MIN
AVG
DEPAR-TURE FROM NORMAL
HEATING
COOLING
TOTAL WATER EQUIV
SNOW-FALL, ICE PELLETS
SNOW, ICE PELLETS OR ICE ON GROUND
AVG SPEED (MPH)
AVG SPEED (KPH)
SKY COVER SUNRISE-SUNSET
WEATHER OCCURENCES
PEAK WIND (KPH) 1
18.3
10.6
16.1
-1.9
-1.0
2.2
0.0
0.00
6.3
10.08 6
S 18.7 2
19.4
12.8
15.0
-3.0
-1.7
3.3
0.09
13.8
22.08
SE 26.2 3
17.2
9.4
-5.2
-2.9
5.6
0.19
27.52 8 5
SW 33.3 4
20.0
13.3
-4.7
-2.6
5.0
0.28
12.3
19.68 9
W 38.6
21.1
1.4
0.8
1.1
11.1
17.76 7
1,2
W 28.3
25.6
16.7
20.6
2.6
8.1
12.96
SW 24.3
23.9
15.2
0.07
S 16.7
0.11
13.2
21.12
S 27.9
22.1
13.9
18.9
0.9
0.5
0.6
4.2
6.72
SE 10.3 10
25.0
12.6
8.88
14.208
S 22.7 11
8.9
15.3
-2.7
-1.5
0.67
19.6
31.36
3,5
S 32.4 12
14.5
10.5
-7.5
-4.2
1.13 T
23.5
37.6
1,3,5
SW 43.8 13
7.2
12.1
-5.9
-3.3
8.3
0.23
14.2
22.72
S 29.8 14
19.3
9.3
0.02
16.8
S 24.5 15
16.5
11.9
19.04
SW 23 16
10.7
6.4
10.24
W 19.2 17
9.5
12.9
-5.1
-2.8
S 27.2 18
16.4
-1.6
-0.9
W 26.3 19
19.1
10.9
15.9
-2.1
-1.2
2.8
4.6
7.36
SW 17.8 20
22.0
18.4
0.4
0.2
7.4
11.84
W 23.1
WEATHER
WEATHER SYMBOLS
CLEAR -SCALE (0-3)
1=FOG
PARTLY CLOUDY (SCALE 4-7)
2=FOG W/VISIBILITY 1/4 MILE OR LESS
CLOUDY (SCALE=8-10)
3=THUNDER
4=ICE PELLETS
5=HAIL
6=GLAZE OR RIME
7=DUSTSTORM OR SANDSTORM
8=SMOKE OR HAZE
9=BLOWING SNOW

5. Create a simplified table for Fly and weather data
Date (June)
Av. Temp.
Hours
Daily ambient thermal energy
(Hours x Ave. Temp
Accumulative Degree Hours - ADH
(Current Daily Ambient Energy + Previous) 20
18.4 15
18.4 x 15 = 276
276 (same as left column) 19
15.9 24
15.9 x 24 = 381.6
= 657.6 18
16.4
393.6 = 17
12.9
309.6
1360.8 16
13.3
319.2
1680
12.8
307.2
1987.2 14
15.0
360
2347.2 13
12.1
290.4
2637.6 12
10.5
252
2889.6 11
15.3
367.2
3256.8 10
16.1
386.4
3643.2 9
18.9
453.6
4096.8 8
4483.2
Military hours of
collection time

6. Fly Life Cycle 21 degrees 240 Hours 21 hours 118 hours 31 hours
Adult
240 Hours
21 hours
Pupa
Eggs
118 hours
31 hours
1st Instar Larva
3rd Instar Larva
26 hours
50 hours
2nd Instar Larva

7. Cumulative degree-hours for each life stage: Species A
Lab Procedure 2, Step 4: By adding all the degree hours for each of the six life stages together, you calculate the cumulative degree hours required for an adult fly to develop at 21°C.
Temp °C
Egg
1st Instar
2nd Instar
3rd Instar
pupa
adult 21
21x21
21 x 31 26 50
118
240
Deg Hrs
441
651
546
1050
2478
5040
Cum. Deg Hrs
= 1092
= 1638
= 2688
= 5166
= 10206
Adult degree-hours = ∑ degree hours at each stage
= cumulative degree hours =

8. Find the Cummunitative Degree Hours Develop to 2nd Instar
Temp °C
Egg
1st Instar
2nd Instar
3rd Instar
Pupa
Adult 21 31 26 50
118
240
Deg Hrs
441
651
546
1050
2478
5040
Cum. Deg Hrs
= 1092
= 1638
= 2688
= 5166
= 10206

9. Find the 1st Number that is Greater than 1638
Date (June)
Av. Temp.
Hours
Daily ambient thermal energy
(Hours x Ave. Temp
ADH
(Current Daily Ambient Energy + Previous) 20
18.4 15
276 19
15.9 24
381.6
657.6 18
16.4
393.6
1051.2 17
12.9
309.6
1360.8 16
13.3
319.2
1680
12.8
307.2
1987.2 14
15.0
360
2347.2 13
12.1
290.4
2637.6 12
10.5
252
2889.6 11
15.3
367.2
3256.8 10
16.1
386.4
3643.2 9
18.9
453.6
4096.8 8
4483.2
1638

10. What is TOD? June 16th – eggs were laid
Add all the hours from June 20th to June 16th
June 20th + June 19th + June 18th + June 17th +June 16th =
111 hours/24 hours
4.625 Days = 4 days and 15 hours
1 day/ .625 hours = 1.6(ratio)
24hours/1.6 = 15 hours

11. New Case – Independent Work
Bodies discovered at 6:00PM on June 20
Insects collected at 8:00PM on June 20
Investigator found 3rd Instar located on the body
What is the TOD of the victim?

12. Create a simplified table weather data
Date (June)
Ave. Temp.
Hours
Daily ambient thermal energy
(Hours x Ave. Temp
Accumulative Degree Hours - ADH
(Current Daily Ambient Energy + Previous) 20 19 18 17 16 15 14 13 12 11 10 9 8

13. Create a Table for Fly Data
Temp °C
Egg
1st Instar
2nd Instar
3rd Instar
Pupa
Adult

14. Determining TOD What day were the eggs laid?____________
Total hours?___________________ = ______
________ Days + _________ Hours

15. DEPAR-TURE FROM NORMAL HEATING COOLING TOTAL WATER EQUIV
DAY
MAX
MIN
AVG
DEPAR-TURE FROM NORMAL
HEATING
COOLING
TOTAL WATER EQUIV
SNOW-FALL, ICE PELLETS
SNOW, ICE PELLETS OR ICE ON GROUND
AVG SPEED (MPH)
AVG SPEED (KPH)
SKY COVER SUNRISE-SUNSET
WEATHER OCCURENCES
PEAK WIND (KPH) 1
18.3
10.6
16.1
-1.9
-1.0
2.2
0.0
0.00
6.3
10.08 6
S 18.7 2
19.4
12.8
15.0
-3.0
-1.7
3.3
0.09
13.8
22.08
SE 26.2 3
17.2
9.4
-5.2
-2.9
5.6
0.19
27.52 8 5
SW 33.3 4
20.0
13.3
-4.7
-2.6
5.0
0.28
12.3
19.68 9
W 38.6
21.1
1.4
0.8
1.1
11.1
17.76 7
1,2
W 28.3
25.6
16.7
20.6
2.6
8.1
12.96
SW 24.3
23.9
15.2
0.07
S 16.7
0.11
13.2
21.12
S 27.9
22.1
13.9
18.9
0.9
0.5
0.6
4.2
6.72
SE 10.3 10
25.0
12.6
8.88
14.208
S 22.7 11
8.9
15.3
-2.7
-1.5
0.67
19.6
31.36
3,5
S 32.4 12
14.5
10.5
-7.5
-4.2
1.13 T
23.5
37.6
1,3,5
SW 43.8 13
7.2
12.1
-5.9
-3.3
8.3
0.23
14.2
22.72
S 29.8 14
19.3
9.3
0.02
16.8
S 24.5 15
16.5
11.9
19.04
SW 23 16
10.7
6.4
10.24
W 19.2 17
9.5
12.9
-5.1
-2.8
S 27.2 18
16.4
-1.6
-0.9
W 26.3 19
19.1
10.9
15.9
-2.1
-1.2
2.8
4.6
7.36
SW 17.8 20
22.0
18.4
0.4
0.2
7.4
11.84
W 23.1
WEATHER
WEATHER SYMBOLS
CLEAR -SCALE (0-3)
1=FOG
PARTLY CLOUDY (SCALE 4-7)
2=FOG W/VISIBILITY 1/4 MILE OR LESS
CLOUDY (SCALE=8-10)
3=THUNDER
4=ICE PELLETS
5=HAIL
6=GLAZE OR RIME
7=DUSTSTORM OR SANDSTORM
8=SMOKE OR HAZE
9=BLOWING SNOW

16. Fly Life Cycle 240 Hours 21 hours 118 hours 31 hours 26 hours 50 hours
Adult
240 Hours
21 hours
Pupa
Eggs
118 hours
31 hours
1st Instar Larva
3rd Instar Larva
26 hours
50 hours
2nd Instar Larva