1. Proteins, Enzymes, and reagent tests.
Jeopardy
Proteins, Enzymes, and reagent tests.

2. What type of monomers make up a polypeptide?
Amino acids
What type of monomers make up a polypeptide?

3. What 4 elements do proteins always contain?
Carbon, Hydrogen, Oxygen, and Nitrogen
What 4 elements do proteins always contain?

4. What does a positive reagent test for protein using Biuret look like?
Any change from blue (Biuret’s color) to purple.
What does a positive reagent test for protein using Biuret look like?

5. What are the monomers that build a protein?
Amino acids
What are the monomers that build a protein?

6. Molecules that repel and do not dissolve in water are described as?
Hydrophobic
Molecules that repel and do not dissolve in water are described as?

7. The blue color disappears (Decolorized)
What does a positive test for vitamin C look like when using Indophenol blue reagent?

8. A carboxyl group and an amino group
What two functional groups are involved in the dehydration synthesis between two amino acids?

9. What is the by-product of dehydration synthesis between 2 amino acids?
Water (H2O)
What is the by-product of dehydration synthesis between 2 amino acids?

10. Enzymes are classified as what type of macromolecule?
Proteins
Enzymes are classified as what type of macromolecule?

11. What is another name for a protein?
A polypeptide
What is another name for a protein?

12. What does it mean when a protein is denatured?
It means its three-dimensional shape has unraveled (unfolded).
What does it mean when a protein is denatured?

13. A protein is a catalyst, meaning it speeds up a chemical reaction.
Define the term enzyme.

14. Water (H2O)
When 2 amino acids are combined through dehydration synthesis what is the by-product?

15. What part of an amino acid is unique to each different amino acids?
The side or “R” group.
What part of an amino acid is unique to each different amino acids?

16. Name this level of protein structure shown below.
The quaternary level
Name this level of protein structure shown below.

17. Define the term polypeptide.
A string of amino acid monomers linked together to form a protein.
Define the term polypeptide.

18. Is this structure an amino acid, lipid, or carbohydrate?
Structural Formula C O OH H N

19. 20 different amino acids
How many different amino acids are used to build all the proteins within the human body?

20. What type of bond is formed between these 2 amino acids?
A peptide bond
What type of bond is formed between these 2 amino acids? C O OH H N C O H N
Leucine
Serine

21. Water (H2O)
What is the by-product released when a peptide bond is formed between these 2 amino acids?
H2O C O OH H N C O H N
Leucine
Serine

22. The primary structure
The diagram below represents a sequence of amino acids in a polypeptide is chain. This level of protein structure is called?

23. False
True or False? Amino acids are made up of glycerol and fatty acids chains (fats).

24. What conditions might cause an enzyme to stop functioning?
Changes in pH and temperature
What conditions might cause an enzyme to stop functioning?

25. A short polypeptide (protein)
What type of molecule is represented by the structural formula seen below?

26. A carboxyl group.
What is the name of the functional group that is circled in the diagram below?
Structural Formula C O OH H N

27. Denaturation
What is the term that describes the unfolding of a protein due to changes in pH or temperature?

28. List 3 functions of a protein.
Immune system function, control chemical reactions, build body structures.
List 3 functions of a protein.

29. How many bonds can carbon make with other atoms.4
How many bonds can carbon make with other atoms.

30. Hydrophillic
Molecule that are attracted to and dissolve well in water are described as?

31. Hydrolysis
When an enzyme helps break sucrose into glucose and fructose water is added. What type of reaction is this?

32. What is the name of this structure?
An amino acid.
What is the name of this structure?
Amino Acid
Fatty acid
Carboxyl group C O OH H N

33. Enzymes lower the _________________ __________ of chemical reactions.
activation energy
Enzymes lower the _________________ __________ of chemical reactions.

34. Any change from blue (benedicts reagent color) to green, yellow, red, orange or brown.
What does a positive test for a reducing sugar, using benedicts reagent, look like?

35. What is the chemical formula for the carboxyl group circled below?
- COOH
What is the chemical formula for the carboxyl group circled below?
Structural Formula C O OH H N

36. A cloudy-white precipitate
What does a positive test for Cl- ions, using Silver Nitrate (AgNO3) reagent, look like?

37. C O OH H N
Side
Group
Amino Group
Hydrogen Atom
Central Carbon
Carboxyl Group
Draw and label the basic (generic) parts of all amino acids. There should be 5 labels.

38. An amino group
What functional group is found in amino acids but not lipids or carbohydrates?

39. Nitrogen
What type of atom (element) is found in amino acids but not lipids or carbohydrates?

40. An amino group.
What is the name of the functional group circled in the structural formula below?
Structural Formula C O OH H N

41. The secondary level of protein structure
The B-pleated and alpha helix structures seen below are considered what level of protein structure?

42. What causes a protein to be denatured?
It is often caused by a drastic change in the proteins environment, such as a shift in pH or a change in temperature.
What causes a protein to be denatured?

43. The tertiary level
A polypeptide chain folded and twisted around itself is considered what level of protein structure? Refer to the diagram.

44. Are enzymes used up in a reaction?No
Are enzymes used up in a reaction?

45. What aspect of a protein will determine its function?
Its structure determines its function
What aspect of a protein will determine its function?

46. A blue-black color change
What does a positive test for starch look like when using Lugols iodine reagent?

47. What is the job of an enzyme in the body?
They speed up chemical reactions
What is the job of an enzyme in the body?

48. What is another name for an enzyme?
A catalyst
What is another name for an enzyme?

49. Activation energy
What is the energy called that is needed to start up a chemical reaction?

50. What is the bond called between amino acids within a protein?
A peptide bond
What is the bond called between amino acids within a protein?