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Cell Organelles.

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Presentation on theme: "Cell Organelles."— Presentation transcript:

1 Cell Organelles

2 Cytoplasm – Transparent gelatinous fluid –
refers to all material between plasma membrane and the nucleus, including 1.organelles (in eukaryotes), 2. cytosol, 3. inclusions Cytosol – largely water with dissolved protein, salts, sugars, and other solutes Liquid Part of the Cytoplasm – fills the spaces where organelles are absent. Aka.. Intracellular fluid, cytoplasmic matrix Contains many organic molecules. Helps keep cell shape along with cytoskeleton.

3 Cytoplasmic organelles – metabolic machinery of the cell
Cytoplasmic Inclusions – chemical substances such as glycosomes, glycogen granules, and pigment Small insoluble particles

4 Cytoplasmic Organelles
Specialized cellular compartments Membranous Organelles Mitochondria, peroxisomes, lysosomes, endoplasmic reticulum, and Golgi apparatus Nonmembranous Cytoskeleton, centrioles, and ribosomes

5 Mitochondria Double membrane structure with shelf-like cristae
Provide most of the cell’s ATP via aerobic cellular respiration Contain their own DNA and RNA

6 Mitochondria Figure 3.17a, b

7 Ribosomes Granules containing protein and rRNA
Site of protein synthesis Free ribosomes synthesize soluble proteins Membrane-bound ribosomes synthesize proteins to be incorporated into membranes

8 Endoplasmic Reticulum (ER)
Interconnected tubes and parallel membranes enclosing cisternae Continuous with the nuclear membrane Two varieties – rough ER and smooth ER

9 Endoplasmic Reticulum (ER)
Figure 3.18a, c

10 Rough (ER) External surface studded with ribosomes
Manufactures all secreted proteins Responsible for the synthesis of integral membrane proteins and phospholipids for cell membranes

11 Signal Mechanism of Protein Synthesis
mRNA – ribosome complex is directed to rough ER by a signal-recognition particle (SRP) Signal-recognition particle (SRP) is released and polypeptide grows into cisternae The protein is released into the cisternae and sugar groups are added

12 Signal Mechanism of Protein Synthesis
The protein folds into a three-dimensional conformation The protein is enclosed in a transport vesicle and moves toward the Golgi apparatus

13 Signal Mechanism of Protein Synthesis
Cytosol Ribosomes mRNA Coatomer- coated transport vesicle Transport budding off Released glycoprotein ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site Sugar group removed Growing polypeptide 1 2 3 4 5 Figure 3.19

14 Signal Mechanism of Protein Synthesis
Cytosol mRNA ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site 1 Figure 3.19

15 Signal Mechanism of Protein Synthesis
Cytosol mRNA ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site Growing polypeptide 1 2 Figure 3.19

16 Signal Mechanism of Protein Synthesis
Cytosol Ribosomes mRNA ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site removed Growing polypeptide 1 2 3 Figure 3.19

17 Signal Mechanism of Protein Synthesis
Cytosol Ribosomes mRNA Released glycoprotein ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site removed Growing polypeptide 1 2 3 4 Figure 3.19

18 Signal Mechanism of Protein Synthesis
Cytosol Ribosomes mRNA Transport vesicle budding off Released glycoprotein ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site Sugar group removed Growing polypeptide 1 2 3 4 5 Figure 3.19

19 Signal Mechanism of Protein Synthesis
Cytosol Ribosomes mRNA Coatomer- coated transport vesicle Transport budding off Released glycoprotein ER cisterna membrane Signal- recognition particle (SRP) Signal sequence Receptor site Sugar group removed Growing polypeptide 1 2 3 4 5 Figure 3.19

20 Smooth ER Tubules arranged in a looping network
Catalyzes the following reactions in various organs of the body In the liver – lipid and cholesterol metabolism, breakdown of glycogen and, along with the kidneys, detoxification of drugs In the testes – synthesis of steroid-based hormones

21 Smooth ER Catalyzes the following reactions in various organs of the body (continued) In the intestinal cells – absorption, synthesis, and transport of fats In skeletal and cardiac muscle – storage and release of calcium

22 Golgi Apparatus Stacked and flattened membranous sacs
Functions in modification, concentration, and packaging of proteins Transport vessels from the ER fuse with the cis face of the Golgi apparatus

23 Golgi Apparatus Proteins then pass through the Golgi apparatus to the trans face Secretory vesicles leave the trans face of the Golgi stack and move to designated parts of the cell

24 Golgi Apparatus Figure 3.20a

25 Role of the Golgi Apparatus
Secretion by exocytosis Extracellular fluid Plasma membrane Vesicle incorporated into plasma membrane Coatomer coat Lysosomes containing acid hydrolase enzymes Phagosome Proteins in cisterna Membrane Vesicle Pathway 3 Pathway 2 Secretory vesicles Proteins Pathway 1 Golgi apparatus Cisterna Rough ER Figure 3.21

26 Role of the Golgi Apparatus
Secretion by exocytosis Extracellular fluid Proteins in cisterna Membrane Vesicle Secretory vesicles Proteins Pathway 1 Golgi apparatus Cisterna Rough ER Figure 3.21

27 Role of the Golgi Apparatus
Secretion by exocytosis Extracellular fluid Plasma membrane Vesicle incorporated into plasma membrane Coatomer coat Proteins in cisterna Membrane Vesicle Pathway 2 Golgi apparatus Cisterna Rough ER Figure 3.21

28 Role of the Golgi Apparatus
Extracellular fluid Plasma membrane Lysosomes containing acid hydrolase enzymes Phagosome Proteins in cisterna Membrane Vesicle Pathway 3 Secretory vesicles Golgi apparatus Cisterna Rough ER Figure 3.21

29 Role of the Golgi Apparatus
Secretion by exocytosis Extracellular fluid Plasma membrane Vesicle incorporated into plasma membrane Coatomer coat Lysosomes containing acid hydrolase enzymes Phagosome Proteins in cisterna Membrane Vesicle Pathway 3 Pathway 2 Secretory vesicles Proteins Pathway 1 Golgi apparatus Cisterna Rough ER Figure 3.21

30 Spherical membranous bags containing digestive enzymes
Lysosomes Spherical membranous bags containing digestive enzymes Digest ingested bacteria, viruses, and toxins Degrade nonfunctional organelles Breakdown glycogen and release thyroid hormone

31 Breakdown nonuseful tissue Breakdown bone to release Ca2+
Lysosomes Breakdown nonuseful tissue Breakdown bone to release Ca2+ Secretory lysosomes are found in white blood cells, immune cells, and melanocytes

32 System of organelles that function to:
Endomembrane System System of organelles that function to: Produce, store, and export biological molecules Degrade potentially harmful substances System includes: Nuclear envelope, smooth and rough ER, lysosomes, vacuoles, transport vesicles, Golgi apparatus, and the plasma membrane PLAY Endomembrane System

33 Endomembrane System Figure 3.23

34 Membranous sacs containing oxidases and catalases
Peroxisomes Membranous sacs containing oxidases and catalases Detoxify harmful or toxic substances Neutralize dangerous free radicals Free radicals – highly reactive chemicals with unpaired electrons (i.e., O2–)

35 Cytoskeleton The “skeleton” of the cell Dynamic, elaborate series of rods running through the cytosol Consists of: 1. microtubules, 2. microfilaments (actin), and 3. intermediate filaments

36 Cytoskeleton Figure 3.24a-b

37 Cytoskeleton Figure 3.24c

38 Microtubules Dynamic, hollow tubes made of the spherical protein tubulin Determine the overall shape of the cell and distribution of organelles involved with motion, mitosis (spindle fibers, and cilia/flagella)

39 Microfilaments Dynamic strands of the protein actin
Attached to the cytoplasmic side of the plasma membrane Braces and strengthens the cell surface – mechanical strength Attach to Cell Adhesion Molecules (CAMs) and function in endocytosis and exocytosis

40 Intermediate Filaments
Tough, insoluble protein fibers with high tensile strength Resist pulling forces on the cell and help form desmosomes Keratin – epithelial cells, hair, nails Part of nuclear envelope Neurofilaments – strengthen axons

41 Motor Molecules - microtubules
Protein complexes that function in motility Powered by ATP Attach to receptors on organelles Transport organelles like mitochondria along axons of neurons.

42 Motor Molecules Figure 3.25a

43 Motor Molecules Figure 3.25b

44 Pinwheel array of nine triplets of microtubules
Centrioles Small barrel-shaped organelles located in the centrosome near the nucleus Pinwheel array of nine triplets of microtubules Organize mitotic spindle during mitosis Form the bases of cilia and flagella

45 Centrioles Figure 3.26a, b

46 Move substances in one direction across cell surfaces
Cilia Whip-like, motile cellular extensions on exposed surfaces of certain cells Move substances in one direction across cell surfaces PLAY Cilia and Flagella

47 Cilia Figure 3.27a

48 Cilia Figure 3.27b

49 Cilia Figure 3.27c

50 Nucleus Contains nuclear envelope, nucleoli, chromatin, and distinct compartments rich in specific protein sets Gene-containing control center of the cell Contains the genetic library with blueprints for nearly all cellular proteins Dictates the kinds and amounts of proteins to be synthesized

51 Nucleus Figure 3.28a

52 Nuclear Envelope Selectively permeable double membrane barrier containing pores Encloses jellylike nucleoplasm, which contains essential solutes

53 Nuclear Envelope Outer membrane is continuous with the rough ER and is studded with ribosomes Inner membrane is lined with the nuclear lamina, which maintains the shape of the nucleus Pore complex regulates transport of large molecules into and out of the nucleus

54 Nucleoli Dark-staining spherical bodies within the nucleus
Site of ribosome production

55 Threadlike strands of DNA and histones
Chromatin Threadlike strands of DNA and histones Arranged in fundamental units called nucleosomes Form condensed, barlike bodies of chromosomes when the nucleus starts to divide Figure 3.29

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