1. The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague
Magdaléna Netuková, Yveta Urbanová,
Josef Šach, Pavel Kuchynka
Ocular Tissue Bank OTB01/The International Eye Bank of Prague, Czech Republic
Dpt. of Ophthalmology Vinohrady Teaching Hospital Prague, Czech Republic
All authors have No finacial interest

2. Aim of the study
To evaluate quality of Pre-Cut corneas for DSAEK prepared in the Interantional Eye Bank of Prague
To analyse possible complications during preparation of the Pre-Cut corneas.
To analyse possible complications after preparation of Pre-Cut corneas

3. Methods - preparation
Pre-Cut corneas were prepared from 30 corneas – not suitable for surgery
All Pre-Cut corneas were preapred in „Clean rooms“ by certified staff members
All Pre-Cut corneas were prepared using standardized method for DSAEK – with Moria microkeratome
Moria microkeratome
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4. No major technical problems
Epithelial abrasion
Mounting
Pachymetry
No major technical problems
during preparation
Cut
Free flap evaluatin
Free flap reposition
Dismounting

5. Methods - Evaluation All Pre-Cut corneas were evaluated using
Slit lamp
Specular microscopy
Selected Pre-Cut corneas were screend for
Microbial comtamination
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6. Results - Slit lamp evaluation
All 30 Pre-Cut corneas were evaluated using slit lamp
In 28 Pre-Cut corneas we found good position of the corneal free flap
In 2 cases we foud dislocation of the free flap into the medium
No obvious stromal or endotehelial defects or opacity were detected
Incorrect position of the free flap
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Correct position of the free flap

7. Results Specular microscopy evaluation
The average endothelial cell censity prior PreCut was 2709±483(SD) cells/mm2,
The average endothelial cell censity after PreCut was 2623±407(SD) cells/mm2
We found no significant endothelial cell loss after preparation.
Corneal endothelium after Pre-Cut

8. Endothelial Cell Density
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9. Resuts - Microbiology
5 randomly selected samples of medium and corneal swaps were microbiologically tested for microbial and fungal contamination – all results were negative
no medium showed signs of bacterial contamination 1 week after preparation of the Pre-Cuts.

10. Summary Preparation Evaluation
In our serie we experienced no major technical problems
The procedure is straight forward with relatively short learning curve
Evaluation
In our serie evaluation of prepared tissue showed high quality and high safety

11. Conclusion
We believe that our eye bank prepares Pre-Cut corneas for lamelar keratoplasty of very high quality and safety
We also believe that in the near future Pre-Cut corneas will represent majority of tissues provided by our eye bank

12. Thank you International Eye Bank of Prague Srobarova 50
Czech Republic
Tel: ,