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Published byGeorgia Stevenson Modified over 6 years ago
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How do police catch criminals just by using a few drops of blood?
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C T T A G C C G T T C G G C A A A T C C A T G A A T T A G G T A
Restriction Enzymes are sequence specific RestrictionEnzyme This one recognizes "AATCGG" and cuts between the T and the C C T T A G C C G T T C G G C A A A T C C A T G A A T T A G G T A
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A T C C A T G A A T T A G G T A C G G C A A C T T A G C C G T T
The Enzyme cuts in a certain way. It leaves "sticky" ends (unpaired bases that need to find a complementary base) RestrictionEnzyme A T C C A T G A A T T A G G T A sticky ends C T T A G C C G T T C G G C A A
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A T C C A T G A A T C G G C A A T A G G T A C T T A G C C G T T
A gene can be inserted as long as it starts and ends with the complementary base pairs! Enzyme A T C C A T G A A T T A G G T A C T T A G C C G T T C G G C A A Another enzyme helps seal the new bases in place
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