1. The Chemokine Receptor CX3CR1 Mediates Homing of MHC class II-Positive Cells to the Normal Mouse Corneal Epithelium
Invest. Ophthalmol. Vis. Sci ;48(4): doi: /iovs
Figure Legend:
Epifluorescence and confocal images of naïve WT (A, B), CX3CR1+/GFP (C), and CX3CR1GFP/GFP (D–F) mouse corneas stained with anti-MHC class II antibody. Low-power epifluorescence image of a WT mouse cornea (A) demonstrates the numerous resident MHC class II+ cells in the peripheral and limbal epithelium (left) in contrast with the low number of cells in the CX3CR1GFP/GFP mouse cornea (D). Intermediate magnification of intraepithelial MHC class II+ cells in the peripheral cornea of WT (B) and CX3CR1GFP/GFP mice (E) demonstrating the differing morphologic appearances of the putative DCs. MHC class II+ eGFP+ cell in the corneal epithelium of an CX3CR1+/GFP mouse (C) displaying typical dendriform morphology compared with the stuntedlike appearance of intraepithelial MHC class II+ cells of CX3CR1GFP/GFP mice (F). DAPI (blue)-stained nuclei (F) to confirm the anatomic position of the MHC class II+ cells in the corneal epithelium.
Date of download: 10/23/2017
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