1. Human Cord Blood Stem Cells Enhance Neonatal Right Ventricular Function in an Ovine Model of Right Ventricular Training 
Ben Davies, MRCS(Eng), PhD, Ngaire J. Elwood, PhD, Shan Li, MS, Fiona Cullinane, FRANZCOG, Glenn A. Edwards, BVSc, MACVSs, Donald F. Newgreen, PhD, Christian P. Brizard, MD 
The Annals of Thoracic Surgery 
Volume 89, Issue 2, Pages e4 (February 2010)
DOI: /j.athoracsur
Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

2. Fig 1
Screening of sheep tissues 1 month after transplantation, by DNA polymerase chain reaction using probes specific for human β-2 microglobulin sequences. β-Actin lanes demonstrate presence of amplifiable DNA. Representative gel from a sheep transplanted at 10 days of age followed by tissue collection 30 days after transplantation. (BM = bone marrow; Kid = kidney; Liv = liver; Lu = lung; LV = left ventricle; RV = right ventricle; Spl = spleen.)
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Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

3. Fig 2
Immunohistochemistry with antibody against human nuclear antigen 1 month after transplantation (transplanted at 1 week of life) in the right ventricle (A), kidney (B), spleen (C), and bone marrow (D). (Human cells indicated by arrowheads.)
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Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

4. Fig 3
Immunohistochemistry for human-specific Alu gene 1 month after transplantation n the right ventricle (A), kidney (B), spleen (C), and bone marrow (D). The arrowheads show human nuclei identified by in situ hybridization for human Alu sequences. (Scale bars indicate 50 μm.)
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Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

5. Fig 4
Confocal microscopy of ovine tissues 1 month after transplantation in the right ventricle showing positivity for human nuclear antigen (HNA) and CD45 (A), the kidney showing CD45-positive human cell (B), the spleen showing cluster of CD45-positive human cells (C), the spleen showing positivity for human CD23 (D), the bone marrow showing positivity for HNA and CD45 (E), and the bone marrow showing positivity for human CD23 (F). (DAPI = 4′-6-Diamidino-2-phenylindole.) (All scale bars indicate 20 μm.)
The Annals of Thoracic Surgery  , e4DOI: ( /j.athoracsur )
Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

6. Appendix Fig 1 Trial design.
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7. Appendix Fig 2 Diagram of operative setup.
The Annals of Thoracic Surgery  , e4DOI: ( /j.athoracsur )
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