1. Application of Ultraviolet/Visible Molecular Absorption Spectrometry
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Application of Ultraviolet/Visible Molecular Absorption Spectrometry
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14
Chapter 14

4. Quantitative Analysis by Absorption Measurement
Scope
Application to absorbing species
Application to non-absorbing species
Procedural Details
Selection of wavelength
Variables that influence absorbance
Cleaning and handling of cells
Determination of the relationship between absorbance and concentration
The standard addition method
Cs = A1CxVx/((A2-A1)*Vx)

5. Standard Addition Method
1.000
0.800
m =
Absorbance
0.600
0.400
b =
0.200
Vs = -6.35
-10.0
0.0
10.0
20.0
Volume standard solution mL

6. Typical Photometric Titration Curves
Volume of titrant
Absorbance
es = ep = 0
et >0
Absorbance
Volume of titrant
es > 0
ep = et = 0
Absorbance
ep > 0
es = et = 0
Volume of titrant
Volume of titrant
Absorbance
es > et > 0
ep= 0
Volume of titrant
Absorbance
et > ep > 0
es = 0
Absorbance
Volume of titrant
es = 0
ep > et > 0