1. SYNTHESIS,CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF
THIADIAZOLE DERIVATIVES

2. INTRODUCTION
Thiadiazole is a 5 membered ring system containing two nitrogen and one sulphur atom.They occur in nature in four isomeric forms viz.1,2,3 thiadiazole;1,2,5 thiadiazole;1,2,4 thiadiazole; and 1,3,4 thiadiazole.
A glance at the standard reference work shows that more work has been carried out on the 1,3,4-thiadiazole than all other isomers combined

3. Members of this ring system have found their way into such diverse application as pharmaceuticals, oxidation inhibitors, cyanine dyes, & metal complexing agents.

4. LITERATURE SURVEY
The literature review showed that the thiadiazole nuclei have antimicrobial , anti inflammatory ,anticancer, anticonvulsant, antidepressant, antioxidant, radioprotective , anti leishmanial activities.
.Synthesis of 2-N-Salicylidene-5-(substituted)-1,3,4-thiadiazole as Potential Antimicrobial Agents1

5. Synthesis of Schiff bases of 2-amino-5-aryl-1, 3,4- thiadiazole And its Analgesic, Anti-Inflammatory , Anti-Bacterial and Anti-Tubercular Activity 2

6. Synthesis, characterization and anticancer activity of 1,2,4-Triazolo [3,4-b]-1,3,4-thiadiazoles on Hep G2 cell lines3

7. Synthesis, characterization and biological activity of some 1, 3, 4-thiadiazol derivatives4

8. Synthesis, Characterization and Antimicrobial Activity of New Thiadiazole Derivatives 5

9. Synthesis of some New Thiosemicarbazide and 1,3,4Thiadiazole Heterocycles Bearing Benzo[b]Thiophene Nucleus as a Potent Antitubercular and Antimicrobial Agents 6

10. Synthesis of Novel Aryloxy Propanoyl Thiadiazoles as Potential Antihypertensive Agents 7

11. Synthesis,characterization andantimicrobial activity of thiadiazole derivatives8
Synthesis and preliminary screening of benzothiazol-2-yl thiadiazole derivatives for anticonvulsant activity9

12. AIM AND OBJECTIVE
From the literature that the thiadiazole function is quite stable, and has inspired chemists to utilize this stable fragment in bioactive moieties to synthesize new compounds possessing biological activities.
Synthesis and characterization of thiadiazole derivatives has been a developing field within the realm of heterocyclic chemistry for the past several decades because of their ready accessibility through synthesis, wide range of chemical reactivity and broad spectrum of biological activity.

13. In view of the ongoing interest is to synthesize nitrogen containing biologically active heterocyclic i.e., thiadiazole derivatives from the different substituted benzoic acids.

14. PLAN OF WORK
Scheme preparation and synthesis of thiadiazole derivatives.
To purify the compounds by appropriate solvent techniques.
The synthesized derivatives will be characterized by I.R , NMR , and Mass spectroscopy.

15. Anti bacterial activity
To evaluate the synthesised derivatives compounds for anti-microbial studies by appropriate methods
Anti bacterial activity
Anti fungal activity
Compilation the the values of biological activites of synthesised compounds with standard and control

16. SCHEME

17. SCHEME-1

18. SCHEME-2

19. SCHEME-3

20. BIOLOGICAL EVALUATION
ANTI BACTERIAL ACTIVITY
CUP PLATE DIFFUSION METHOD
The cup plate method depends up on diffusion of antibiotic from a cup through a solidified agar layer in a petridish or petri plates to an extent such that growth of added microorganism is prevented entirely in a zone around cup or cylinder containing a solution of antibiotic.
Test organisms used for anti bacterial activity:
Escherichia coli.

21. ANTI FUNGAL ACTIVITY Asperagillus niger
CUP PLATE TECHNIQUE.
The cup plate technique described by Hugo and Russel (1984) was employed in studying the antifungal activity.
The cup plate method depends up on diffusion of antibiotic from a cup through a solidified agar layer in a petridish or petri plates to an extent such that growth of added microorganism is prevented entirely in a zone around cup or cylinder containing a solution of antibiotic.
Test organisms used for anti fungal activity:
Asperagillus niger

22. REFERENCE
1.Jumat salimon*, nadia salih†, hanan ibraheem† and emad yousif† asian journal of chemistry vol. 22, no. 7 (2010), Alok Pandey1*, Dhansay Dewangan1, Shekhar Verma2, Achal Mishra3, Ravindra Dhar Dubey 2CODEN( USA): IJCRGG ISSN : Vol. 3, No.1, pp Jan-Mar Dhanya Sunil1, Arun M Isloor2* and Prakash Shetty3 Scholars Research Library Der Pharma Chemica, 2009, 1(2): Arvind kumar singh*1, mahfooz lohani2 and umesh pratap singh1 .

23. 5. Pooja Mullick,* Suroor A. Khan, Surajpal Verma, and Ozair Alam. Bull. Korean Chem. Soc. 2010, Vol. 31, No DOI /bkcs S.L. Vasoya, D.J. Paghdar, P.T. Chovatia, and H.S. Joshi* Islamic Republic of Iran 16(1): (2005). 7.Amarish B. Samel and Nandini R. Pai* Journal of the Chinese Chemical Society, 2010, 57,