1. Altered Social behavior in brain tumor rats model
Lusine Hovhannisyan, Manushak Avagyan, Vahe Mkrtchyan, Elya Minasyan, Tamara Harutyunyan, Mariam Movsisyan, Senik Matinyan
Introduction
Objective
Different studies evidence that rats act socially and value social relations, whereas brain tumors have deleterious effect on brain neurological and cognitive functions.
The purpose of this study is to evaluate the social behavior of rats implanted with brain tumor. Cyclosporine immunosuppressed rats with sarcoma 105 injected in hippocampal region are used as brain tumor model and specific changes in social behavior were tested.
Methods
Adult albino rats weighting 100+/- 5 grams were immunosuppressed with cyclosporine 25mg/kg i/p 48h prior to surgery and during all days of the experiment[1].
Rats were anesthetized with Nembutal 40 mg/kg i.p. and placed on stereotaxis frame[2].
The tumor cells were injected in hippocampus (AP –3.5 mm, ML 3.0 mm, DV 2.3 mm)[3]. The control group was injected with isotonic saline solution.
Social behaviour was assessed on day 7 of the experiment using the social interaction test[4].
The rats were sacrificed on day 10 of experiment and morphological analysis was conducted to validate tumor location.
Results and discussion
Animals with brain tumor showed significantly higher social and empathetic behavior.
This results may be due to lesion induced impairment and stress.
Conclusion
We conclude that this model can be successfully used for assessment for social behavior which can be feasibly measured.
References
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