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Molecular quantification of bacteria from respiratory samples in patients with suspected ventilator-associated pneumonia M. Clavel, O. Barraud, V. Moucadel, F. Meynier, E. Karam, M.-C. Ploy, B. François N. Pichon, P. Vignon, R. Droual, C. Duchiron, J. Vignaud, D. Chainier, M. Mattei, A. Sommabere, E. Mercier, C. Le Brun, A. Desachy, C. Garandeau, M. Rodrigue, M. Lacroix, S. Prudent, M.-A. Jestin, J. Yugueros-Marcos M. Clavel, O. Barraud, V. Moucadel, F. Meynier, E. Karam, M.-C. Ploy, B. François N. Pichon, P. Vignon, R. Droual, C. Duchiron, J. Vignaud, D. Chainier, M. Mattei, A. Sommabere, E. Mercier, C. Le Brun, A. Desachy, C. Garandeau, M. Rodrigue, M. Lacroix, S. Prudent, M.-A. Jestin, J. Yugueros-Marcos Clinical Microbiology and Infection Volume 22, Issue 9, Pages 812.e1-812.e7 (September 2016) DOI: /j.cmi Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 1 Concordance results between quantitative PCR and conventional culture on bronchoalveolar lavage for Staphylococcus aureus, Pseudomonas aeruginosa and Haemophilus influenzae. (a) The quantified loads of each specimen (Log 10 scale) were plotted for both methods (culture method considered as reference standard method in x-axis and molecular method in y-axis). The number of represented specimens is written between brackets for aggregated specimens (same loads measured by both methods). Regarding conventional culture, the results outside the quantification limits (<102 or >107) are expressed as equal to 0 or 107, respectively. (b) Associated receiver operating characteristic curves. Blue: concordant results, red: discordant results. Clinical Microbiology and Infection , 812.e1-812.e7DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 2 Concordance results between quantitative PCR and conventional culture on endotracheal aspirate for Staphylococcus aureus, Pseudomonas aeruginosa and Haemophilus influenzae. (a) Quantified loads of each specimen (Log 10 scale) were plotted for both methods (culture method considered as reference standard method in x-axis and molecular method in y-axis).The number of represented specimens is written between brackets for aggregated specimens (same loads measured by both methods). Regarding traditional culture, the results outside the quantification limits (<103 or >107) are expressed as equal to 0 or 107, respectively. (b) Associated receiver operating characteristic curves. Blue: concordant results, red: discordant results Clinical Microbiology and Infection , 812.e1-812.e7DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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