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Biomedical Optics KAIST

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Presentation on theme: "Biomedical Optics KAIST"— Presentation transcript:

1 Biomedical Optics Lab @ KAIST
“Understanding, diagnosis, and treating human disease with light” PI: YongKeun Park Department of Physics, KAIST Understanding disease Cellular imaging Super resolution lens Photobleaching-free imaging Optical Field Control Optical Field Measurement Tissue tomography Diagnosis disease Control multiple scattering Biomaterial-Photon Interaction Active holographic optical tweezers Treating disease Optical control of complex objects

2 Super-resolution live-cell imaging based on diffusion-assisted FRET
(Forster resonance energy transfer) In collaboration with MC Choi, TY Yoon, MW Kim, Wondo Heo, Hyochirl Rhee (KAIST) S-Y. Cho et al, Scientific Reports, 2013

3 a b Research Highlight #1: super-resolution microscopy … sample
Target structure labeled with donor Conventional imaging sample obj. lens excitation filter dichromic mirror lens mirror CCD camera LED illumination emission filter donor b Localizing blinked subset of donor via diffusion-driven FRET Super-resolution imaging acceptors … t1 t2 t3 S-Y. Cho et al, Scientific Reports, accepted

4 a b Intensity (a.u.) Position (nm) Widefield dSOFI dSOFI
+deconvolution 1 µm b -400 -200 200 400 1 Intensity (a.u.) Position (nm) widefield: nm dSOFI: nm dSOFI(decon): nm S-Y. Cho et al, Scientific Reports, accepted

5 a b c i i ii ii iii iii iv iv Widfield dSOFI 1 μm 5 μm Widfield dSOFI
Wide field dSOFI 0.8 Intensity (a.u.) 0.6 0.4 0.2 1 2 3 4 5 6 7 Position (nm) S-Y. Cho et al, Scientific Reports, accepted

6 0 min 5 min 10 min 15 min 5 μm a b S-Y. Cho et al, Scientific Reports, accepted

7 Acknowledgement Funding
KAIST Dr. Youngchan Kim Dr. Jaeduck Jang Junghoon Park Kyoohyun Kim Hyeonseung Yu Jaehwang Jung Kyorae Lee Suresh group, MIT Monica Diez Ming Dao Prof. Subra Suresh Levine Group, UCLA Mark L. Henle Tatiana Kurunikova Alex J. Levine Univ. Twente, Netherland E. van Putten Allard P. Mosk Weizmann Institute, Israel Thorsten Auth Samuel Safran Nir Gov National University of Singapore Rajesh Chandramohanadas Peter Rainer Preiser KAIST Prof. Yonghoon Cho Dr. Choonghyun Park Prof. Mahn Won Kim Dr. Hyunjoo Park Dr. Junwoo Jung Prof. Yonghee Lee Spectroscopy Lab., MIT Timothy R. Hilman Zahid Yaqoob Kamran Badizadegan (Harvard) Ramachandra R. Dasari Prof. Michael Feld Asan Hospital, Korea Hyoeun Shim Sungsoo Jang JaeHyun Lim Funding KAIST, Samsung Electronics, Korea government NRF (Brain Korea 21 project, Basic Research Lab project, NRF-2012R1A1A ), Chungam Foundation

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