1. Laboratory safety (Practical)
Physiology
232 BMS
Dr/Nahla Yacout
2016/2017

2. Objectives List dangers that may be present in labs
List the general safety measures
List the safety measures regarding fires, during glassware handling & during handling chemicals
List the safety measures during heating substances & after experiments
Identify the laboratory first aid kit that must be present in labs

3. Laboratory hazards
There are many dangers that may be present in labs, including:
Broken glass
Corrosive reagents
Inflammable chemicals
Poisonous fumes
Gas leakage
Electrical hazard

5. Safety measures
Safety measures that must be taken inside labs: General safety measures 1. All written & verbal instructions should be carefully followed 2. Lab coat has to be worn 3. Keep hands away from face, eyes, mouth & body while using chemicals 4. Gases, blood, urine, CSF & other biological fluids should be handled with great care as they are important source of infection 5. Chemical work involving irritating chemicals should always be conducted under hoods with adequate ventilation 6. Electrical heaters should be checked frequently 7. Eating & drinking in labs must be avoided

6. Safety measures

7. Safety measures regarding fire safety
1. Smoking should be prevented
2. Burning match sticks should not be thrown in waste baskets
3. Any gas leakage should be properly reported
Safety measures during glassware handling
1. Never handle broken glass with your bare hands
2. Examine glassware before each use, to be sure that it is not cracked
3. Don’t immerse hot glassware in cold water

8. Safety measures during handling chemicals
1. All chemicals in lab must be considered dangerous & so
must be handled carefully
2. Don’t taste or smell any chemical
3. Clear labeling of all the bottles containing chemicals & reagents
should be done & their hazards should be noted on the label
4. Check the label on the chemical bottle twice before removing it
5. Bottles containing chemicals or reagents should be held with both hands, & should not be carried by holding their necks
6. Take only as much chemical as you need

9. 7. Never return unused chemicals into their original containers 8
7. Never return unused chemicals into their original containers 8. Never pipette by mouth 9. Precautions must be taken while handling carcinogenic substances Bottles containing these substances must be labeled as (Carcinogenic) Plastic gloves must be used while handling these substances

10. Safety measures during heating substances
1. Heated glassware remains very hot for long time, & so they should
be set aside in a place to cool, & picked up with caution
2. Never look into a container that is being heated

11. Safety measures after experiments
1. Clean all the glassware you have used, & put them on a shelf in
a proper place
2. Clean the table
3. Put all chemicals back to their places

12. In case of fire Laboratory first aid kit that must be present in labs:
Cotton wool
Roller bandage
Disinfectant solution
Acetic acid
5% sodium carbonate
In case of fire
1. Blanket should be used to put off small fires
2. (Fire extinguishers) must be used for large fires
3. Water should not be used
4. While trying to escape from fire, stay close to the floor & crawl by covering mouth