1. Results of bachelor thesis
Synthesis of Core-Shell - and Core-Shell-Shell and their in vivo applications on Diatoms
Results of bachelor thesis
David Harting

2. Outline Outline Introduction Objectives Results and Conclusion 2

3. I. Introduction unicellular, eukaryotic, photosynthetic algae
can be found in every sea and fresh water habitat
responsible for 20% of the fixation of carbon
cell walls consist of amorphous silicates with complex structures in the nano range
cell walls formed by biomineralization
A. Falciatore, C. Bowler, Annual Reviews of plant Biology 2002, 53, 109 – 30.
M. Hildebrand, Chemical Reviews 2008, 108, 4855 – 4874.
N. Kröger, N. Poulsen, Annual Review of Genetics 2008, 42, 83 – 107.

4. I. Introduction nános = greek: dwarf
use of diatoms in nanotechnologie because of their complex structure
diatoms are already used as templates
creation of new nanostructures through the metabolic absorption of nanoparticles through diatoms
need of core-shell-nanoparticles  combination of different properties
J. Toster, K. Swaminathan Iyer, R. Burtovyy, Journal of American Chemical Society 2009, 131, 8356 – 8357.
S. Fouilloux, A. Désert, O. Taché, O. Spalla, J. Daillant, A. Thill, Journal of Colloid and Interface Science 2010, 346, 79 – 86.

5. II. Objectives Synthesis of core-shell-particles
growing of diatoms on nanoparticle solutions
characterization of grown diatoms

6. III. Results and conclusion
TEM-image of with larger SiO2-cores.
TEM-image of after reconditioning and use in growth attempts.

7. III. Results and conclusion
Day 16: left S. turris at low concentration of Au-NP; right S. turris at high concentration of Au-NP.
Day 16: left D. brightwellii at low concentration of Au-NP; right D. brightwellii at high concentration of Au-NP.

8. III. Results and conclusion
Left S. turris sample with PDDA, day 4;
right S. turris sample with PVP, day 3.
Left D. brightwellii sample with PDDA, day 4 ;
right D. brightwellii sample with PVP, day 3.

9. III. Results and conclusion
REM-image of extracted samples; left S. turris with PDDA, right S. turris with PVP.

10. III. Results and conclusion
further experiments to clarify the state of how the gold nanoparticles are bound to the diatoms
use of other stabilizer substances, with a focus on compatibility with the diatoms
pursue of the synthesis of nanoparticles
search for suitable methods for the seperation of the algae and nanopaticles in solution

11. Thank you for your attention.