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Nucleic Acid Labeling Guide
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Choose labeling method based on Application:
Matrix binding(e.g. subtractive hybridization) & In situ hybridization & Cellular localization & Blot hybridization Does the probe need to be labeled at the end? Yes No What kind of label is needed? biotin fluorophores amines 5'-EndTag Kit for end labeling at 5' end MB-9001 3'-EndTag Kit for end labeling at 3' end MB-9002 PHOTOPROBE Biotin Kit SP-1000 PHOTOPROBE (Long Arm) Biotin Kit SP-1020 FastTag Labeling Kit MB-8000 PHOTOPROBE Amine Kit SP-1070 biotin fluorophores amines What kind of label is needed? Oligonucleotide labeling Comparative genomic hybridization
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Choose labeling method based on Type of probe:
siRNA mRNA Does the probe need to be labeled at the end? Yes No What kind of label is needed? biotin fluorophores amines 5'-EndTag Kit for end labeling at 5' end MB-9001 3'-EndTag Kit for end labeling at 3' end MB-9002 PHOTOPROBE Biotin Kit SP-1000 PHOTOPROBE (Long Arm) Biotin Kit SP-1020 FastTag Labeling Kit MB-8000 PHOTOPROBE Amine Kit SP-1070 biotin fluorophores amines What kind of label is needed? under 100bp over 100bp How long is your probe? DNA
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Labeling nucleic acids with haptens, fluorochromes, or ligands
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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FastTag Labeling System
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Labeling DNA with biotin using heat or light
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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PHOTOPROBE Biotin System
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Dotting and detecting labeled probes on a membrane
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Dot Blot
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Dot Blot
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Dot Blot
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Dot Blot
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Dot Blot
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Dot Blot
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