1. BACTERIA: Get to know ‘em. A “G” Science Production Copyright 2001
KINGDOM MONERA
BACTERIA:
Get to know ‘em.
A “G” Science Production
Copyright 2001

2. Anton van Leeuwenhoek
( )
Discovered Bacteria
Father of the Modern Microscope

3. Characteristics of Bacteria
Simplest Living Organisms
Single Celled: Prokaryote (no true Nucleus)
Reproduce by Fission (dividing into 2)
Found Everywhere
Nutrition
Chemotrophs - eat by absorbing chemicals
Phototrophs - make food by photosynthesis

4. Bacteria Structure

5. CLASSIFICATION 1. By shape a) Cocci (Spherical)
b) Bacillus (Rod-shaped)
c) Spirillum (Spiral Cell)

6. Cocci
Spherical

7. Bacillus
Rod-Shaped

8. Spirillum
Spiral Cell

9. Spirillum

10. Spirillum

11. CLASSIFICATION Arrangements Staphylo- = Clusters Strepto- = Chains
Prefixes
Staphylo- = Clusters
Strepto- = Chains
Diplo- = Twos

12. Staphylococcus aureus

13. Streptococcus pyogenes

14. Diplococcus

15. Anthrax (Bacillus anthracis)

16. CLASSIFICATION 2. By Cell Structure Simple Staining - tells shape
We use 1 stain.
Grams Staining - to see cell wall structure

17. Gram-negative rods

18. Aerobic,
Gram-positive rods

19. Aerobic, Gram-positive cocci

20. Anaerobic, Gram-positive rods

21. Anaerobic,
Gram-positive cocci

22. Growth Requirements Food - Water - Warmth - (Store in Incubator)
agar contains all Nutrients
Water -
agar, powder & water mixture
Warmth - (Store in Incubator)
optimum temp. for most rapid growth is:
degrees C
degrees F

23. Culturing To provide all the requirements for growth
Usually requires hours for growth
Each dot (.) that appears is a COLONY, growth started from 1 or more bacteria cells

24. Visible colonies of bacteria in a petri plate.

26. The Good With The Bad BAD = GOOD =
Bacteria can cause disease and infections. Ex. Pneumonia, scarlet fever, TB, Anthrax
GOOD =
Decompose materials “Nature’s Recycler” Make plastics, pesticides, oil eating bacteria

28. I hope you enjoyed your introduction into the wonderful world of….
BACTERIA

29. Place a slide with a bacterial smear on a staining rack.
STAIN the slide with crystal violet for 1-2 min.
Pour off the stain.
Note: fingers stain Gram-positive - use forceps!
Flood slide with Gram's iodine for 1-2 min.
Pour off the iodine.
Decolourize by washing the slide briefly with acetone (2-3 seconds).
Wash slide thoroughly with water to remove the acetone - do not delay with this step.
Flood slide with safranin counterstain for 2 min.
Wash with water.
Blot excess water and dry in hand over bunsen flame.

30. Summary Slide
The Good With The Bad