1. Garlic Sulphur Biochemistry
Workpackage Four
Garlic Sulphur Biochemistry
Partner 2:
Horticulture Research International
Laurence Trueman, Brian Thomas,
Linda Brown & Brian Smith

2. To identify developmental control points for CSO synthesis
Objective: 2
To identify developmental control points for CSO synthesis
Milestones Feb
Analysis of second-year field experiment completed
Whole plant labelling studies completed

3. Hydroponic Printanor A=50% total sulphur (d. 102, end March)
B=Start bulbing (d. 130, 20 Apr)
C=Start of senescence of root & leaf (d. 170, end May)
D=Harvest (d. 230, end Jul)
A B C D

4. Hydroponic Printanor Distribution and remobilization
of sulphur taken up early
* * * * * * * * * * * every 14d
Distribution and remobilization
of sulphur taken up late
* * * * * * * * * * * every 14d
A B C D
34S
32S

5. What we want to know When does nutrient uptake from the medium stop
When does bulb filling start
What proportion of S (and N) comes from the roots, leaves and hydroponic medium
The form of the S in the root and leaf

6. Hydroponic data
Bulb Index
Fresh weight
Dry Weight

7. To isolate and characterise alliinase cDNA clones
Objective: 3b
To isolate and characterise alliinase cDNA clones
Milestones Feb
Start Expression Analysis

8. Garlic Alliinase Expression relative to total rRNA content

9. Comparison of DNA derived polypeptide sequences
Peptide 1 = Published, 2 = Al48 & 3 = Al41

10. Comparison of Peptide sequences
Al48 has 98.9% identity to other known garlic cDNA’s
Al41 has only the N-terminal 50% of the peptide in common with other alliinases
The N-terminal portion of this peptide is likely to direct this peptide to the vacuole - Does it act as a storage protein?

11. Is it real?
Al Al41

12. Clustering relationship of Messidrome alliinase PCR fragment sequences
l=Leaf Clone b=Bulb Clone

14. mRNA Expression - Method