1. From: A New Human Primary Epithelial Cell Culture Model to Study Conjunctival Inflammation
Invest. Ophthalmol. Vis. Sci ;54(10): doi: /iovs
Figure Legend:
Establishment of epithelial cell culture. Two disaggregation methods were studied (A). Briefly, the enzymatic method consisted of a 2 hour incubation with dispase and 5 minutes incubation with trypsin/EDTA. The combined method included a mechanical scraping between both incubations. Viability of recovered cells was significantly higher with the enzymatic method (P = ) than with the combined one (n = 9). Two purification techniques were assayed (B). After 2 hours of preplating all the fibroblasts were attached. After 5 minutes of contact with trypsin, all the fibroblasts were detached, whereas most of the epithelial cells still remain attached to the culture surface (n = 3). ***P ≤
Date of download: 10/25/2017
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