1. Diffusion

3. Selectively Permeable Membrane

4. Animal Cell
(Normal Saline 0.9%)
= solute

5. Activity 1 Diffusion experiment Methylene blue MW 320g/mole
Potassium permagnate MW 150 g/mole
Form Hypothesis
T0 initial diameter T60 final diameter
Calc. rate of diffusion
Room temp

6. Table 1. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp
Time
MB RT
PP RT T0
10 mm
T60
20 mm
10 mm/hr

7. Table 2. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp Group Data
MB RT
PP RT 1
10mm/hr
8 mm/hr 2 3 4 5
Ave SD

8. Activity 2 Osmosis through semipermeable membrane
Create 4 dialysis bags, filled with unknown concentration sucrose solutions (10 ml)
Weigh bags and then immerse in 10% sucrose solution (50 ml)
Reweigh bags after 30 minutes of experiment
Identify the bags containing 0%,10%, 20%, and 40% of sucrose, based on weight changes.

9. Unknown solutions of sucrose
(0, 10, 20 & 40 %)
10 ml of unknown sucrose into dialysis bag

10. Dialysis Tubing Open tubing with water Tie knot at one end
Fill with one of the unknown solutions (10ml)
Tie other end

11. 10 ml of unknown sucrose into dialysis bag

12. Weigh pre and post

13. Place dialysis bag in 50 ml 10% sucrose for 30 minutes