1. Forensic Science International
Looking at flubromazolam metabolism from four different angles: Metabolite profiling in human liver microsomes, human hepatocytes, mice and authentic human urine samples with liquid chromatography high-resolution mass spectrometry 
Ariane Wohlfarth, Svante Vikingsson, Markus Roman, Mikael Andersson, Fredrik C. Kugelberg, Henrik Green, Robert Kronstrand 
Forensic Science International 
Volume 274, Pages (May 2017)
DOI: /j.forsciint
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2. Fig. 1
Structures of flubromazolam and related benzodiazepines midazolam, alprazolam, triazolam, pyrazolam and flubromazepam.
Forensic Science International  , 55-63DOI: ( /j.forsciint )
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3. Fig. 2
Combined extracted ion chromatograms showing flubromazolam and metabolites in hydrolyzed (continuous line) and non-hydrolyzed (gray line) mouse urine and two representative human urine specimens. Arrows indicate the release of the phase I metabolite/parent from the phase II conjugates. Bold labeled metabolites represent major differences between mouse and human: whereas the mouse metabolism proceeds to dihydroxylated metabolites, the human metabolism rather conjugates monohydroxylated metabolites and the parent itself with glucuronic acid.
Forensic Science International  , 55-63DOI: ( /j.forsciint )
Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

4. Fig. 3
Product ion spectra and proposed structures of flubromazolam and metabolites. No product ion spectra were obtained for M1 and M4 due to low intensity.
Forensic Science International  , 55-63DOI: ( /j.forsciint )
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5. Fig. 4
Proposed structures and formulas, exact masses, mass measurement errors and electron configuration (EE=even-electron, OE=odd-electron configuration) for characteristic fragment ions present in the product ion spectrum of flubromazolam. The most intense ions are highlighted in bold.
Forensic Science International  , 55-63DOI: ( /j.forsciint )
Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

6. Fig. 5
Proposed structures and formulas, exact masses, mass measurement errors and electron configuration (EE=even-electron, OE=odd-electron configuration) for characteristic fragment ions present in the product ion spectrum of α-hydroxy-flubromazolam. The most intense ions are highlighted in bold. Structures for common fragment ions described for the analogous metabolites α-hydroxy-alprazolam and α-hydroxy-triazolam are shown at the right for purpose of comparison (see further explanation in the text).
Forensic Science International  , 55-63DOI: ( /j.forsciint )
Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

7. Fig. 6
Proposed structures and formulas, exact masses, mass measurement errors and electron configuration (EE=even-electron, OE=odd-electron configuration) for characteristic fragment ions present in the product ion spectrum of 4-hydroxy-flubromazolam. The most intense ions are highlighted in bold.
Forensic Science International  , 55-63DOI: ( /j.forsciint )
Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions