1. Ag and Au nanoparticles (NPs)
UFN
Ag and Au nanoparticles (NPs)

2. Nanomaterials 2 one dimension < 100 nm (1 m)
Amin, M. T. et al. Adv. Mater. Sci. Eng. 2014,

3. Nanomaterials 3

4. Au and Ag NPs 4 100 nm Au nanoparticles Au nanoshapes Ag nanocarrot
Ag nanorice
Au nanocages
Au nanoframes
Liang, H.-Y. et al. Front. Phys. 2016, 11,
Sajanlal P. R. et al. Nano Rev. 2011, 2, 5883.

5. Nanoparticles (NPs) 5
Sajanlal P. R. et al. Nano Rev. 2011, 2, 5883.

6. Příprava nanočástic 6

7. Příprava nanočástic 7
Supercritical Atomization
Jet-milling

8. Význam povrchových atomů8
Repeat 24 times
For example, 5 cubic centimeters – about 1.7 cm per side – of material divided 24 times will produce 1 nanometer cubes and spread in a single layer could cover a football field.
Source: Clayton Teague, NNI

9. Význam povrchových atomů9
podíl atomů na povrchu
< 3,5 nm > 10 nm

10. Analýza nanočástic 10
SEM
TEM
AFM
DLS

11. AuNPs – history 11 4th – 5th century B.C.
Middle Ages – for the diagnosis of syphilis (the test is not reliable)
1718 – use of boiled starch in drinkable gold preparation noticeably enhanced its stability
1857 – Michael Faraday reported the formation of deepred solutions of colloidal gold by reduction of an aqueous solution of chloroaurate (AuCl4-) using phosphorus in CS2 (a two-phase system).
Daniel, M.-C. et al. Chem. Rev. 2004, 104, 293–346.

12. AuNPs preparation 12 Turkevitch in 1951 Brust-Schiffrin in 1994
Daniel, M.-C. et al. Chem. Rev. 2004, 104, 293–346.
ImageService/Articleimage/2013/CC/c2cc35720h/c2cc35720h-f1.gif

13. AuNPs applications 13 Human chorionic gonadotropin (hCG)
Immunoglobulin G (IgG)
antibody-antigen interactions
Yeh, C.-H. et al. Sensors 2014, 14, 16148–16158.

14. AgNPs applications 14

15. AuNPs 15
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

16. Capping agents 16
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

17. Capping agents 17
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

18. AuNP, -SH ligands 18
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

19. AuNP, -SH ligands 19
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

20. Coupling of peptides 20
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

21. Bioconjugation of AuNPs21
arginine–glycine–aspartic acid
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

22. Bioconjugation of AuNPs22
Kim, Y.-H. et al. Small 2011, 7, 2052–2060.

23. Bioconjugation of AuNPs23
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

24. Doxorubicin conjugates24
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

25. Doxorubicin conjugates25
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

26. Doxorubicin conjugates26
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

27. AuNP and CuAAC 27
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

28. Modification of Au nanorods28
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

29. Luciferase-AuNP 29
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

30. Noncovalent conjugation30
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

31. Noncovalent conjugation31
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

32. AuNP-oligonucleotide32
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

33. AuNP-oligonucleotide33
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

34. AuNP-transferrin 34
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.

35. AuNP-transferrin 35
(1) Sapsford, K.-E. et al. Chem. Rev. 2013, 113, 1904–2074.