1. 6.11-15 culturing Microorganisms
Microbiology

2. 6.11 Microbial Growth in Communities
Ecological Associations
Symbiosis
Parasitism-Pathogenic
Mutualism-Both Benefit
Commensalism-One Benefits, Other unharmed

3. 6.11 Microbial Growth in Communities
Biofilms
Cells adhere to each other on a surface
Five Stages
Initial attachment
Irreversible attachment
Maturation I
Maturation II
Dispersion

4. 6.12 Control of Microbial Growth
Joseph Lister
Very Important in many fields
Methods: Heat, Chemicals, Irradiation, High Pressure and filtration
Sterilization-kills everything
Disinfectants-destroy cell wall or interfere w/ metabolism
Antiseptics-Used on living tissue to reduce infection
Antibiotics-Broad spectrum and targeted
Antibacterials-Bactericidal & Bacteriostatic

5. 6.12 Control of Microbial Growth
Rate of Microbial Death

6. 6.12 Control of Microbial Growth
Relative Resistance
Endospores-Most resistant
Infections-Difficult to treat
Horizontal gene transfer
Antibiotic Resistance-Gram neg.
Staphylococcus aureus
Fungal-Most susceptible

7. 6.13 Mechanisms of Microbial control
Alteration of Membrane Permeability
Antimicrobial Drugs
Polymyxin & gramicidin

8. 6.13 Mechanisms of Microbial control
Damage to Nucleic Acids
Bacteriostatic antibiotics
Broad Spectrum
Stops Bacterial Reproduction
Tetracycline, sulfonamides, Spectrinomycin

9. 6.14 Physical Antimicrobial Control
Heat
High Pressure
Autoclave
Radiation
Low Temperatures
Desiccation
Freeze-Drying
Osmotic Pressure
Salt & Sugar
Filtration nm

10. 6.15 Chemical Antimicrobial control
Disinfection
Bitrex
High-Med-Low
Germicides
Ethanol, Hydrogen Peroxide, Iodine

11. 6.15 Chemical Antimicrobial control
Antiseptics (living) disinfectants (non-living)
Air-Bleach & glycols
Liquid-Alcohols & Aldehydes
Oxidizing Agents-Chlorine & Oxygen

12. 6.15 Chemical Antimicrobial control
Biological control
Maggot Therapy
Probiotics
Antibiotics