1. Prepared By: Heba Mohamed Fahmy, PhD
Nigella sativa as an anti-inflammatory and promising remyelinating agent in the cortex and hippocampus of experimental autoimmune encephalomyelitis-induced rats
Prepared By: Heba Mohamed Fahmy, PhD
Lecturer at Biophysics Department, Faculty of Science, Cairo University, Egypt

2. Introduction and aim of the study
What is multiple sclerosis (MS)?
What is experimental autoimmune encephalomyelitis (EAE)?
What is Nigella sativa (N. sativa)?
The aim of the present study was to investigate the protective and therapeutic
effects of the whole N. sativa seeds (2.8 gm / kg of animal weight) in the EAE-induced rats.

3. Animals were randomly divided into 4 groups
Materials and Methods
Animals were randomly divided into 4 groups
“Control” group
“EAE” group
“EAE + N. sativa” group
“N. sativa + EAE” group
Open field test
Daily clinical scoring
Body weight
Disease monitoring
Histopathological examination
Ultrastructural examination
Oxidative stress parameters

4. Nigella sativa suppresses inflammation in the EAE-induced groups
Results
Daily clinical scoring
Body weight
Open field test
Histopathological examination
Nigella sativa suppresses inflammation in the EAE-induced groups

5. Ultrastructural examination
Cortex
Hippocampus

6. Oxidative stress parameters
Cortex

7. Hippocampus

8. Conclusions
The whole N. sativa seeds attenuate the clinical signs, suppress inflammation and lead to marked remyelination in EAE-induced Wistar rat.
N. sativa seeds could provide a promising agent effective in both the protection and treatment of EAE even when the treatment started after the appearance of first clinical signs.

9. Thank you for your attention
Any questions?