1. MeV Ion Microbeams and Radiation Biology at the University of Surrey
Geoff Grime, Norman Kirkby, Karen Kirkby and Roger Webb
University of Surrey
Guildford, U.K.

2. Guildford

3. Surrey involvement in CELLION
Four academic staff:
Dr Geoff Grime
Dr Karen Kirkby
Prof. Roger Webb
Dr Norman Kirkby
Three Research Centres:
Centre for Nuclear and Radiation Physics
Ion Beam Centre
Process and Information Systems Engineering Research Centre

4. The University of Surrey Ion Beam Centre
Established as a centre for ion implantation and semiconductor device development
1978
Established as a UK National Facility for Ion Beam Applications
1980 – 1990s
Development of ion implantation from 2 keV to 2 MeV using a variety of accelerators
Development of ion beam analysis (esp. RBS) using 2 MV Van de Graaff and 10 mm scanning microbeam
Some biological applications (mainly relating to diffusion in skin and hair)
2001
Installation of new 2MV tandem accelerator and OM microbeam system
2002
GWG transfers from Oxford: New initiatives in beamline development and biological application programme
2004
CELLION
UK Research Network on Biological Applications of MeV Microbeams (starting in May 2004)

5. Ion Beam Centre staff in CELLION
Dr Geoff Grime
Techniques for focusing MeV ion microbeams
Development of PIXE analysis
Trace element microanalysis of cells and tissue using PIXE and RBS
Dr Karen Kirkby
Ion beam modification of materials
Coordination of UK Research Network for ion beam applications in biological sciences
Prof. Roger Webb
Modelling of ion-solid interactions
Director of Ion Beam Centre

6. Ground plan of Ion Beam Centre (Feb 2004)

7. 2MV Tandetron accelerator

8. RBS chamber and microbeam

9. New external beam (Feb 2004)

10. New developments planned (short term):
Major initiative to enhance spatial resolution
New beamline
100nm for full current modes
<20 nm for low current modes
Develop new techniques
Precisely positioned single ions
High aspect ratio nano-fabrication
Enhance the facilities
State of the art sample positioning on all beamlines including existing microbeam
High quality video imaging to assist in sample positioning and location
Remote access and control

11. Proposed nanobeam line

12. Nanobeam development Requirements < 100nm resolution (full current)
< 20 nm resolution (single ion mode)
Issues to address:
Slit/gas scattering
Long working distance (to aid design of general purpose sample chamber)
Accurate scanning
Single ion capability (Precisely Positioned Single Ions)
Cell irradiation in horizontal geometry
Progress to date
Graduate student working on beam optics
Investigation of two-stage system

13. Multi-stage lens system
High quality intermediate image
Aperture at intermediate image removes halo from slit scatter
High overall demagnification so large object aperture (to minimise slit scatter)
Final stage can have long working distance to simplify design of target chamber

14. Horizontal (in vacuum) nanobeam – design concept

15. Long term plans

16. Side view of vertical beamline
Ion focusing system suspended in vertical column isolated from rest of building
Workstation (microscope,sample stage, detectors etc.) on upper level of extension
Remote operation (via internet)
Concrete columns
Existing accelerator hall
90 degree magnet
0 degree beamline

17. Cell Cycle Model University of Surrey
Dr Norman Kirkby
Developing techniques for modelling cell cycle dynamics in conjunction with Gray Cancer Institute and other academic and clinical organisations
Mitosis
DNA Synthesis
First Gap
Phase
Second Gap
CelCyMUS

18. Cell Cycle Model University of Surrey
Modelling
&
Experiments
Novel Bioreactors
Reactor Control
Non-linear Dynamics
Medical Applications

19. Further work on cell cycle modelling
Dr Norman Kirkby
Models of DNA damage and repair
Models of phase extension while damage is repaired
Models of mitotic and apototic death
Models of cell signalling during the above
Multi-scale modelling of radiotherapy: gene expression to patient outcome

20. Interaction with CELLION
Ph.D. student working at the interface between cell cycle modelling and experimental irradiation
Postdoctoral RA working on the design and commissioning of the horizontal nanobeam
Sharing experience – networking!

21. MeV Ion Microbeams and Radiation Biology at the University of Surrey
Geoff Grime
et al.
University of Surrey
Guildford, U.K.