1. Figure 1. Representative mismatched amplification mutation assay polymerase chain reaction amplicons for detection of 16u>c rrs mutation in 14 Mycobacterium tuberculosis isolates. (A) The 233-bp wild type rrs allele amplicons obtained from rrs-16 wt and rrs-RM primers (arrow). (B) The 233 bp-16u>c rrs mutant allele amplicons obtained from rrs-16 mt and rrs-RM primers (arrow). Numbers on the left are DNA sizes (bp). Lane M: GeneRuler 100 bp DNA Ladder (Fermentas, Vilnius, Lithuania); lane 1: no template control; lane 2: rrs wild type allele control (H37Rv); lane 3: rrs-16u>c mutant allele control; lane 4–17: clinical M. tuberculosis isolates.
Figure 1. Representative mismatched amplification mutation assay polymerase chain reaction amplicons for detection of 16u>c rrs mutation in 14 Mycobacterium tuberculosis isolates. (A) The 233-bp wild type rrs allele amplicons obtained from rrs-16 wt and rrs- RM primers (arrow). (B) The 233 bp-16u>c rrs mutant allele amplicons obtained from rrs-16 mt and…
Tuberc Respir Dis Apr;80(2):