1. اجابة السؤال الاول

2. 1- Exons and Introns

3. 2- Shine Dalgorne sequence

4. 3- Exonuclease III

5. 4- Restriction endonuclease

6. 5- Alkaline phosphatase

7. اجابة السؤال الثانى

8. 1- Okasaki fragment

9. 2- Excision repair

10. 3- tRNA

11. 4- Lac operon

12. 5- Real-Time PCR Advantages
Real-time PCR enables continuous monitoring of the appearance of the amplified product during the reaction.
For measuring levels of a transcript, the process begins with RNA not DNA.
Advantages
It is the ease and speed of detection of the product
Quantifiable
Not need gel electrophoresis
Useful in diagnostic applications of PCR

13. 5- Real-Time PCR

14. 6- Genomic Libraries
A genomic library is a set of recombinant clones that contains the entire DNA present in an individual organism.
An E. coli genomic library, for example, contains all the E. coli genes.

15. 6- cDNA libraries

16. 7- Studying a transcriptome by: 7- Studying a Proteome by:
Sequence analysis
Serial analysis of gene expression (SAGE)
Microarray analysis
DNA chip analysis
7- Studying a Proteome by:
Protein electrophoresis
Mass spectrometry

17. 8- Shotgun and Contig
Shotgun: insertion of random fragments of DNA into a vector.
Contig: DNA sequence built up from a number of smaller overlapping sequences, during a sequencing project

18. 8- Chromosome walking