1. اساس اندازه گیری در سل کانتر
اساس اندازه گیری در سل کانتر

2. سل کانتر Orphee

3. Electrolytes solutions allow current to pass from them شرکت سپهر آنالیز
Like
ISOTON III

5. A red cell passes through RBC aperture
The CBC principle
A red cell passes through RBC aperture
Red Blood Cell
Sensing Zone
Oscilloscope
Oscilloscope

6. A red cell passes through RBC aperture
The CBC principle
A red cell passes through RBC aperture
Red Blood Cell
Sensing Zone
Oscilloscope
Oscilloscope

7. A red cell passes through RBC aperture
The CBC principle
A red cell passes through RBC aperture
Red Blood Cell
Sensing Zone
Oscilloscope
Oscilloscope

8. A White cell passes through WBC aperture
The CBC principle
A White cell passes through WBC aperture
Neutrophil
Oscilloscope
Sensing Zone
Oscilloscope

9. A White cell passes through WBC aperture
The CBC principle
A White cell passes through WBC aperture
Neutrophil
Oscilloscope
Sensing Zone
Oscilloscope

10. A White cell passes through WBC aperture
The CBC principle
A White cell passes through WBC aperture
Neutrophil
Oscilloscope
Sensing Zone
Oscilloscope

11. Passage of a RBC & Plt through aperture
RBCs + -
PLT
Cross section of
RBC aperture
(60x50 micron)
Oscilloscope screen

12. Passage of a RBC & Plt through aperture
RBCs + -
PLT
Cross section of
RBC aperture
(60x50 micron)
Oscilloscope screen

13. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

14. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

15. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

16. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

17. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

18. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

19. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

20. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

21. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

22. System without sweep flow
RBCs recirculating + -
RBC
PLT
Oscilloscope screen
Sensing Zone

23. System with sweep flow + - RBCs pushed away by diluent RBC PLT
Oscilloscope screen
Sensing Zone
Diluent stream

24. System with sweep flow + - RBCs pushed away by diluent RBC PLT
Oscilloscope screen
Sensing Zone
Diluent stream

25. System with sweep flow + - RBCs pushed away by diluent RBC PLT
Oscilloscope screen
Sensing Zone
Diluent stream

26. System with sweep flow + - RBCs pushed away by diluent RBC PLT
Oscilloscope screen
Sensing Zone
Diluent stream

27. System with sweep flow + - RBCs pushed away by diluent RBC PLT
Oscilloscope screen
Sensing Zone
Diluent stream

28. Sweep Flow technology
RBCs swept away from sensing zone
Diluent stream

29. Pulse editing Cell flows through centre of aperture
Good Pulse
Diluent stream

30. Pulse editing Non central cell flow
Pulse to be
edited
Diluent stream

31. Coincidence Correction
Pulse to be
edited
Diluent stream

32. Applying Thresholds to separate Plt from RBC
20 fl
2 fl
Base line
Platelets
RBC of various
sizes

33. RBC histogram 256 channel high resolution RBC histogram
Main RBC population
RBC doublets
256 channel high resolution RBC histogram
display cell population data between 24 and 350 fl

34. PLT histogram and curve fitting
A fitted curve accurately reflecting the collected data is displayed between 2 and 35 fl
Raw data
Fitted Curve displayed up to 35fl
PLT histogram is based on 64 channels

35. PLT curve fitting
The Platelet count is derived from the number of cells under the fitted curve beyond the 35fl display limit extending up to 70 fl
Raw data
Fitted Curve extend up to 70 fl to report very large PLT count

36. PLT curve fitting
The curve fitting process allows more accurate counts when platelets of larger than 20 fl are present

37. PLT curve fitting
The curve fitting process allows more accurate counts when platelets of larger than 20 fl are present
PLTs
RBCs

38. CBC WBC histogram 256 channel high resolution WBC histogram
Lymphocyte
Monocyte
Granulocyte
256 channel high resolution WBC histogram
display cell population data between 35 and 450 fl

39. Coulter WBC histogram
Mono
Lymp
Neut
Baso
Eos

40. Hgb Measurement Derivation Signal ADC Processor WBC Bath HGB Lamp
Absorbance= log 10 ( VR / Vs )
Where VR = Reference voltage
Vs = Sample voltage
HGB Sensor &
PRE-AMP
Sample