1. Designed lignocellulosic-based films
for tunable physico-chemical and spectral properties
Aguié-Béghin V, Muraille L., Paës G, Chabbert B

2. Objective & Strategy
Physical , chemical , and cytochemical studies of LC deconstruction and properties
Determining and isolating individual features that limit LC deconstruction, and govern LC properties : role of polymer interactions
Designing Lignocellulose-based self-assemblies (films)
Assemblies of plant cell walls which mimic some of the interactions, composition, physical states found in plant cell wall
Nanostructured LC with variable properties

3. Designing bioinspired LC assemblies
Elementary building brick:
Cellulose: Cellulose nanocrystals, nanofibrils (CNCs, CNF)
(from ramie, flax, hemp fibers)
Hemicelluloses: feruloylated arabinoxylans, xylan, glucomannan
Lignin: model lignin (DHP) and lignin from biorefinery
Ramie CN
Flax CN
Aspect ratio ~ 30-50
Mw ~3000 g mol-1
Ralph et al., 2007

4. Non-covalent and covalent
Designing bioinspired Lignocellulose-based films
Nanostructuration of bio-inspired LC assemblies:
Modulation of polymer interactions (non covalent/ covalent linkages):
- physical blending
- polymerization or cross-linking of phenyl propane units via enzymatic reaction
or chemical oxidation
Hemicellulose
(Xylan or Feruloylated
ArabinoXylan (FAX))
Non cellulosic polysaccharide- lignin (LCC)
Non-covalent and covalent
Interactions
Hydrogen-bonds
van der Waals and Hydrophobic interactions
Ether bonds
Cellulose Nanocrystals
(CN)
Hemicellulose - CN
Bioinspired assemblies complexity

5. Main results Bioinspired LC model systems
Nanostructuration of LC polymers generate bioinspired systems with enhanced physico-chemical properties :
Modulation of interactions (covalent, non-convalent) between polymers
– Impact on physico-chemical (water sorption and spectral properties),
– Impact on mechanical properties,
– up-grading value of lignin in nanocomposites
Relevant templates which emphasize the relative and quantitative importance of selected parameters (LC network, protein) on probes mobility:
– Characterization and understanding of probe mobility in lignocelluloses
– Model to predict macromolecular design of efficient lignocellulolytic enzymes