1. TUDA Progress

2. TUDA Sample Collection
Cohort
Completed bloods and questionnaires
Data entry
Completed
Dexa scans
Cognitive
1465
1360
(not double)
Bone
771
635
641
Hypertension
1800
512
1749

3. TUDA Laboratory Analysis
Blood Metabolite
Cognitive (N=1465)
Completed Results
Bone (N= 771)
Hypertensive (N=1800)
Serum folate
1286
564
1764
Red cell folate
Serum total B12
1670
Serum holoTC
900
100
Plasma tHcy
1300
500
Plasma MMA
Plasma B6 (PLP)
1200
200
1400
Red cell B2 (EGRAC)
1350
Serum D vitamers
1100
Serum Cross Laps
N/A
1750
Serum Osteocalcin
Serum BAP
Plasma Bone TRAP

4. TUDA DNA Extractions Cognitive (N=1385 ) DNA extracted Bone (N= 641)
Hypertensive (N= )
1073
800
Prepared DNA is stored in two tubes;
Tube 1 contains 100 µL of normalised DNA at a concentration of approximately 100 ng/µl.
Tube 2 contains the original purified stock DNA at variable concentrations.

5. Laboratory Analysis for NANS (N=1145)
Blood Metabolite
Final Results
Serum folate
1125
Red cell folate
1129
Serum total B12
1124
Plasma tHcy
1130
Red cell B2 (EGRAC)
400
Buffy coat for DNA
Extracted DNA
1127
Lower numbers than 1145 for each metabolite are due to missing serum, plasma or buffy coat samples that were not included in the shipments from UCD/UUC