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12. Aliquot extracts for analysis
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Step 6
Data analysis
Sample plant tissue
Step 1
Weigh Sample Aliquots
Step 2
Extract Samples
Step 3
Aliquot extracts for analysis
and sample back-ups
Step 4
ELISA Analysis
Step 5

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21. Design DNA/Line specific Protein specific Protein specific
PCR ELISA Strips
Design DNA/Line specific Protein specific Protein specific
Requirements Sophisticated Moderate Easy
Assay time days days min
Sample cost $ $ $7-10
Availability Published/Testing Co Diagnostic Co Diagnostic Co.
Validation JRC (qualitative) JRC/USDA USDA
Application Qualitative/semi-quant Qual/semi/quant Qual/ test/ compliance with
threshold
Limitations Basic pH, heat, heat, extraction, Same as ELISA false pos, false neg reference materials pro-zone, dynamic sys Protein dependent sample/test
Sensitivity ~>0.1% GMO ~>0.3% GMO ~1% GMO
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