1. Volume 122, Issue 2, Pages 376-386 (February 2002)
Aminopeptidase N is involved in cell motility and angiogenesis: Its clinical significance in human colon cancer 
Hiroki Hashida, Arimichi Takabayashi, Michiyuki Kanai, Masashi Adachi, Keiichi Kondo, Nobuoki Kohno, Yoshio Yamaoka, Masayuki Miyake 
Gastroenterology 
Volume 122, Issue 2, Pages (February 2002)
DOI: /gast
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2. Fig. 1
Dose-dependent effect of mAb MH8-11 on cell penetration. Cell penetration was determined with HT1080-penetrating cells into the lower chamber of a Transwell. A constant inhibitory effect was produced by an mAb MH8-11 concentration of 5 μg/mL.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Effect of mAb MH8-11 on the motility of HT1080 cells as determined by a phagokinetic assay using the gold colloid method. (A) HT1080 cell motility in medium containing mouse IgG (5 μg/mL). (B) HT1080 cell motility in medium containing MH8-11 (5 μg/mL).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Effect of mAb MH8-11 on EC migration. The number of migrated HUVECs through the filter coated by reconstituted basement membrane was counted. HUVEC migration was inhibited more significantly by functional mAb MH8-11 (5 μg/mL; P < 0.001).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Effect of mAb MH8-11 on HUVEC tube formation (original magnification, 200×). (A) HUVEC tube formation in medium containing mouse IgG (5 μg/mL). (B) HUVEC tube formation in medium containing mAb MH8-11 (5 μg/mL).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Effect of mAb MH8-11 on HUVEC tube (A) width and (B) length. HUVECs were plated onto Matrigel in the presence of mAb MH8-11 (5 μg/mL). Each experiment showed a similar and significant decrease in the presence of mAb MH8-11 (P < and < 0.001, respectively).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

7. Fig. 6
Western blotting analysis of APN/CD13 expression. Arrowhead indicates 165-kD molecular weight.
Gastroenterology  , DOI: ( /gast )
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8. Fig. 7
Immunohistochemical staining of human colon cancer tissues using the avidin-biotin-peroxidase complex procedure (original magnification 150×). (A) APN/CD13-positive staining of colon cancer. (B) APN/CD13-negative staining of colon cancer.
Gastroenterology  , DOI: ( /gast )
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9. Fig. 8
(A) Overall survival of 114 colon cancer patients according to their tumor APN/CD13 status. (B) Overall survival of 42 node-positive colon cancer patients according to their tumor APN/CD13 status.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

10. Fig. 9
(A) Disease-free survival of 114 colon cancer patients according to their tumor APN/CD13 status. (B) Disease-free survival of 42 node-positive colon cancer patients according to their tumor APN/CD13 status.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions