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Identification of necrophagous fly species using ISSR and SCAR markers

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Presentation on theme: "Identification of necrophagous fly species using ISSR and SCAR markers"— Presentation transcript:

1 Identification of necrophagous fly species using ISSR and SCAR markers
Lin He, Sibao Wang, Xuexia Miao, Hong Wu, Yongping Huang  Forensic Science International  Volume 168, Issue 2, Pages (May 2007) DOI: /j.forsciint Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Amplification of genetic DNA in five necrophagous fly species using primer (CA)6GT. 1, 2: Lucilia sericata, adult; 3, 4: L. sericata, second instar larva; 5, 6: L. sericata, third instar larva; 7, 8: Aldrichina grahami, adult; 9, 10: Chrysomya megacephala, adult; 11, 12: C. megacephala, third instar larva; 13, 14: Musca domestica, adult; 15, 16: Parasarcophaga crassipalpis, adult. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Amplification of different individuals using primer (CA)6GT. 1, 3, 5, 7, 9, 11, 13, 15: L. sericata, second instar larva; 2, 4, 6, 8, 10, 12, 14, 16: L. sericata, third instar larva. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

4 Fig. 3 Agarose gel electrophoresis of the amplification products obtained from species-specific SCAR markers; 1, 2 L. sericata, adult; 3, 4: A. grahami, adult; 5, 6: C. megacephala, adult; 7, 8: P. crassipalpis, adult; 9, 10: M. domestica, adult (molecular size markers (lane M) in base pairs are indicated on the left of each panel. (A) shows the SCAR marker for C. megacephala, (B) shows the SCAR marker for P. crassipalpis and (C) shows the SCAR marker for M. domestica). Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

5 Fig. 4 Reliability test of SCAR marker for M. domestica in three populations collected in Shanghai. The three locations are about 30km from each other. Letters with number in the figure showed the different individual. Forensic Science International  , DOI: ( /j.forsciint ) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions


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