1. VIABILITY ASSAYS

2. What is Viability and Viability assays ?

3. How we check viability? Tetrazolium assay
Lactate dehydrogenase determination
Adenylate energy charge
Rate of protein or nucleic acid synthesis
Colony forming assay

4. Tetrazolium assay Colometric based
Used tetrazolium dye like MTT [3-(4,5-dim ethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] Lactate dehydrogenase
Enzyme based
Measure by spectrophotomectric assay

5. Pyruvate +NADH+H+ LDH lactate + NAD+​ Index based ,
Pyruvate +NADH+H+ LDH lactate + NAD+​
​Adenylate energy charge
Index based
The energy charge= ATP+ 0.5ADP
​ ATP+ADP+AMP
Interconversion of 3 adenylate
AMP ADP ATP ​

6. Rate of protein or nuclic acid synthesis
Rate of protien or DNA synthesis
Radioactive amino acids or nucleotides
Measure by tritiated thymidine Colony forming assay
Viable cells foam colony