1. Schematic outline of MeDIP
Schematic outline of MeDIP. Genomic DNA is sheared into approximately 400 to 700 bp using sonication and subsequently denatured. Incubation in 5-mC antibodies, along with standard immunoprecipitation (IP), enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to 1 of 2 DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question. (From
Source: Methods of Molecular Analysis, The Basic Science of Oncology, 5e
Citation: Tannock IF, Hill RP, Bristow RG, Harrington L. The Basic Science of Oncology, 5e; 2016 Available at: Accessed: October 27, 2017
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