1. Parts of the microscope

2. Compound Light Microscope
Used to observe small objects
Magnifies images up to 2000X their size

3. Different parts of a microscope
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5. SCI.9-12.B [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation

6. Optic or body tube – connects eyepiece to objective lens
Revolving nosepiece – turns objectives
Type of objectives – they all magnify at different strengths
3. Scanning (the shortest one) -- magnifies 4x
B(4). Low power objective – magnifies 10x
C(5). High power objective (longest one) -- magnifies 40x

7. M(6). Stage clips – holds slide in place
J(7). Diaphragm – controls how much light is on slide – 2 types are a. wheel and b. lever
K(8). Light
A(9)Eyepiece – look through it and magnifies 10 x
H (10)Arm – carry microscope with
D (11)Stage – holds slide or object being looked at

8. F(12). Coarse focus – sharpens image by moving stage
G(13). Fine focus knob – sharpens image, see depth and adjusts for differences in eyesight
E(14). Base – supports microscope

9. Total magnification – how many times what you are looking at is magnified
T.M. = eyepiece x objective
Scanning x 4 = 40
Low power – 10 x 10 = 100
High power – 10 x 40 = 400
Oil immersion – 10 x 100 = 1000

10. SCI.9-12.B [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation

11. Resolution is how clear an image is
Resolution is how clear an image is. As magnification goes up, resolution goes down. We say they are inversely related.

12. Revolving nosepiece
Eyepiece
Body tube
Objective
Coarse adjustment
Clip
Fine adjustment
Arm
Condenser
Stage
Iris diaphragm
Condenser control knob
Mirror
Base

14. Observing Cells Light microscope
Can observe living cells in true color
Magnification of up to ~1000x
Resolution ~ 0.2 microns – 0.5 microns

15. Observing Cells Electron Microscopes
Preparation needed kills the cells
Images are black and white – may be colorized
Magnifcation up to ~100,000
Transmission electron microscope (TEM)
2-D image shows inside
Scanning electron microscope (SEM)
3-D image shows outside

16. SEM
TEM

17. 25 Amazing Electron Microscope Images https://www. youtube. com/watch

18. Video on Electron Microscope https://www. youtube. com/watch

20. Coarse focus
Objective
Stage
Body Tube
Fine focus
Stage Clips
Diaphragm