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Napins, 2S albumins, are major allergens in oilseed rape and turnip rape  Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,

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Presentation on theme: "Napins, 2S albumins, are major allergens in oilseed rape and turnip rape  Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,"— Presentation transcript:

1 Napins, 2S albumins, are major allergens in oilseed rape and turnip rape 
Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali, PhD, Nisse Kalkkinen, PhD, Timo Reunala, MD, PhD, Kristiina Turjanmaa, MD, PhD, Timo Palosuo, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 117, Issue 2, Pages (February 2006) DOI: /j.jaci Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Purification of immunoreactive proteins from oilseed rape (A) and turnip rape (B). Marked fractions from gel filtration (smaller picture) of oilseed rape and turnip rape extracts were further subjected to cation exchange chromatography (larger picture). Four fractions from oilseed rape (I-IV) and 2 fractions from turnip rape (I and II) were selected for further analyses. Absorbance was measured at 280 nm. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 SDS-PAGE and Coomassie blue staining (A) of cation exchange and RP chromatographic purified fractions of oilseed rape and turnip rape (loaded 2 μg per lane; lane 3, 1.2 μg) and IgE immunoblot analysis (B) to cation exchange fractions with pool of sera from 8 patients. C, Atopic control; lanes 1 through 4, oilseed rape fractions I through IV, respectively; lanes 5 and 6, turnip rape fractions I and II, respectively. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 MALDI-TOF mass spectrum of purified napins from oilseed rape fraction II (A) and turnip rape fraction II (B). [M+2H]2+, Doubly charged forms of the proteins. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 IgE binding to purified oilseed rape fraction II (A) and turnip rape fraction II (B) proteins of sera from sensitized children (n = 72) and atopic control subjects (n = 72) in different age groups by means of ELISA. Cut-off level for a positive response (mean OD + 3 SD of control subjects) is indicated by a horizontal line. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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