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Improving performance of nanoscale ultrasound contrast agents using N,N- diethylacrylamide stabilization  Reshani H. Perera, PhD, Hanping Wu, MD, PhD,

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Presentation on theme: "Improving performance of nanoscale ultrasound contrast agents using N,N- diethylacrylamide stabilization  Reshani H. Perera, PhD, Hanping Wu, MD, PhD,"— Presentation transcript:

1 Improving performance of nanoscale ultrasound contrast agents using N,N- diethylacrylamide stabilization  Reshani H. Perera, PhD, Hanping Wu, MD, PhD, Pubudu Peiris, PhD, Christopher Hernandez, BS, Alan Burke, BS, Helen Zhang, BS, Agata A. Exner, PhD  Nanomedicine: Nanotechnology, Biology and Medicine  Volume 13, Issue 1, Pages (January 2017) DOI: /j.nano Copyright © 2016 Elsevier Inc. Terms and Conditions

2 Figure 1 (A) Schematic diagram of lipid and pluronic-stabilized nanobubbles with interpenetrating crosslinking biodegradable polymer N,N-diethyl acrylamide (NNDEA) and N,N-bis(acryoyl) cystamine (BAC)crosslinking network. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

3 Figure 2 Size distribution of the CL-PEG-NB obtained by (A) DLS and (B) qNano; (C) Concentration distribution of CL-PEG-NB obtained from qNano; (D) STED image of Alexa Fluor 488 tagged CL-EG-NB; (E) Surface morphology of the CL-PEG-NB and normal PEG-NB visualized using scanning electron microscopy (SEM). Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

4 Figure 3 (A) Representative ultrasound images of nanobubbles in vitro, showing both custom-made tissue phantom area and the contrast agent area. (B) Enhanced in vitro stability of CL-PEG-NB compared to original PEG-NB over 1h (inset) and 24h as measured as a function of time. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

5 Figure 4 Ultrasound images showing the contrast in each organ and the tumor after the bubble injection as a function of time. Regions of interests (ROIs) delineate the liver, kidney, and subcutaneous tumor. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

6 Figure 5 Ultrasound intensity of each organ and the tumor after injecting (A) PEG-NB; (B) CL-PEG-NB; (C) Normalized ultrasound intensity of each organ at the initial phase after peak enhancement (n=3). Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

7 Figure 6 Tumor nanobubble and microbubble kinetics measured with FMT. (A) FMT images show the accumulation of Vivo680-tagged CL-PEG-NB and Vivo680 tagged PEG-MB in tumor at 1h post-injection; (B) Fluorescence intensities of tumor after application of above treatments as a function of time. In each case, CL-PEG-NB perform better than the control. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

8 Figure 7 Fluorescence intensities of each organ (liver, kidney, heart) 5 and 60min of post-application of Vivo680-CL-PEG-NB and Vivo680-PEG-MB. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

9 Figure 8 (A) Representative fluorescence images showing the bubble distribution in tumor (green). Alexa 488-tagged CL-PEG-NB showed higher extravasation compared to the Alexa 488-tagged PEG-MB. MB confined to the tumor vasculature (red); (B) Representative image showing the extravasation of CL-PEG-NB into the tumor beyond the tumor vasculature; (C) The signal intensities of bubble and vessel expressed as the percentage of total cells of tumor tissues. Nanomedicine: Nanotechnology, Biology and Medicine  , 59-67DOI: ( /j.nano ) Copyright © 2016 Elsevier Inc. Terms and Conditions

10 Nanomedicine: Nanotechnology, Biology and Medicine 2017 13, 59-67DOI: (10.1016/j.nano.2016.08.020)
Copyright © 2016 Elsevier Inc. Terms and Conditions

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