1. Professional Internship for PhD Students (PIPS)
Shaun Calvert
Professional Internship for PhD Students (PIPS)
TS/MS Speke

2. Introduction Who I am My time at Elanco
MRes ‘Post Genomic Technologies’ – Liverpool
3 year PhD, ‘Genetics of Ageing’ – Liverpool
My time at Elanco
What I got
What Elanco got

3. Master of Research
Identification of caloric restriction mimetic using gene expression data.
Testing the effectiveness of these drugs in C.elegans
Identify the mechanism of action.

4. PhD Successful ageing in the long lived Naked Mole Rat (NMR)
Cell culture NMR vs Mouse skin fibroblasts

5. PhD – Methods Microarray – Masters work RNAseq Cell culture
Data analysis – small and large scale
Dose studies
ELISAs
Flow cytometry
qPCR – validation and detection

6. Elanco What: Development scientist Why: New experience
Develop and refine manufacturing processes
Why: New experience
Protein science
Industry vs academia
Big Pharmaceutical company
When: start of PhD year three (final year)

7. What I got New lab experience Experience with Industry HPLC
Downstream processing
capture chromatography, buffer exchange, protein refold
DoE
Other (cell lysis, Western)
Experience with Industry
GMP
Tours
Conference calls
Learning plan

8. What I contributed Data New staining method Expertise Helping hands
qPCR and primer design
Helping hands
Poster days
House keeping
Fairshare

9. Questions?