1. stress
Tau Pathology
&
Alzheimer’s Disease
xiao zitian

2. GCs (glucocorticoids) TAU hyperphosphorylation
Key words:
GCs (glucocorticoids) 1
TAU hyperphosphorylation 2
Aβ (Amyloid β) 3

3. Method & Material Animal Stress model Infusion Behavioral Immunoblot
Age:14 months
Standard environmental conditions
Animal
Daily
Chronic stressors: 1.hypertonic saline; 2.overcrowding(1 h);
3.confined environment
(30 min);
4.vibrating/rocking platform (1 h)
Stress
model
Intracerebroventri-cular infusions
(solubilized
A β 1-40 or vehicle)
Sg: subcutaneous GC injections
Infusion
1.Spatial reference memory(tested by
Morris water maze)
2.Assessment of reversal learning(collected by video tracking system)
Behavioral
test
1.Western blot analysis
2.immunocytochemistry
Immunoblot

4. Results & Discussion
Sotiropoulos I, Catania C, Pinto L G, et al. Stress acts cumulatively to precipitate Alzheimer's disease-like tau pathology and cognitive deficits[J]. The Journal of Neuroscience, 2011, 31(21):
1.induce ：chronic stress & abnormal hyperphosphorylation
2.overexpression: stress and GCs increased the expression of a number of kinases in the hippocampus and PFC of animals
3.activator: stress and/or GC activated pERK1/2, pGSK3, p35 (the cdk5 activator),pJNK, and CaMKII in the hippocampus and PFC