1. Metiner Tosun, Ph.D. Faculty of Medicine, Dept. of Internal Medicine
Systems Biology for Translational Medicine
Prospective Project Partnership Meeting
DIfferentIal expressIon Pattern of TrP Ion Channel Genes In CarcInogenesIs
Metiner Tosun, Ph.D.
Faculty of Medicine,
Dept. of Internal Medicine

2. Field of Study / Technical Experience
Keywords: cancer, aging, calcium, functional genomics
General Information: Transient Receptor Potential (TRP) calcium ion channels have distinct and significant roles in normal as well as in disease states. Differential expressional pattern of TRP genes appear to have diagnostic and/or treatment potential in vascular aging and cancer development. Functional genomics data from our ongoing study show that altered expression pattern of these genes may be operational in phenotypic switch of “cancer stem cells” into tumor cells. Therefore, drug-resistant cancer cells could be specifically targeted based on their TRP expression profiles.

3. Projects
Ongoing: Functional genomics analysis of store-operated calcium entry in phenotypic differentiation of Huh-7 human hepatocellular carcinoma cell lines. TUBITAK 113S399. (PI)
Recent publications on liver cancer:
Selli C, Pearce DA, Sims AH, Tosun M. Differential expression of store-operated calcium and proliferation-related genes in hepatocellular carcinoma cells following TRPC1 ion channel silencing. Mol Cell Biochem, 420(1-2):129-40, (doi: /s )
Selli C, Erac Y, Kosova B, Erdal SE and Tosun M. Silencing of TRPC1 regulates store-operated calcium entry and proliferation in Huh7 hepatocellular carcinoma cells. Biomed Pharmacother, 71:194–200, (doi: /j.biopha )
Selli C, Erac Y, Tosun M. Simultaneous measurement of cytosolic and mitochondrial calcium levels: Observations in TRPC1-silenced hepatocellular carcinoma cells. J Pharmacol Toxicol Methods. 72:29-34, (doi: /j.vascn ).
Completed:
Functional genomics analysis of miRNA expressional profile associated with post-transcriptional control of TRPC ion channel genes in human primary aortic smooth muscle cells. TUBITAK 110S096, (PI)
Investigation of functional expression profile of TRPC gene family in carcinogenesis via Post-Transcriptional Gene Silencing. TUBITAK 108S072, (PI)
Pharmacological and molecular approaches in controlling Capacitative Calcium Entry in vasospasm development. TUBITAK 104S568, September (PI)
The role of differantial expression pattern of Trpc gene family in rat vascular smooth muscle during aging. TUBITAK 103S176, June (PI)
Investigation of telomerase activity and maintenance in vascular smooth muscle cell proliferation developed following balloon angioplasty in rabbit iliac artery. TUBITAK 104S058, (Co- PI)

4. Project Idea / Contribution Fields
Proposed project title:
Specific: Expression pattern of Ca2+-related genes in neurodegenerative disease (i.e. Alzheimer’s) models. Institutional: Genomic pattern recognition of diseases, Pharmacogenomics, etc.
Techniques and equipment* :
Real time changes in intracellular calcium levels (QM8-2005, PTI).
Vascular reactivity (MP100, BioPac)
Real-Time Cellular Analysis (Excelligence RTCA MP and DP, Roche)
Immunofluorescence microscopy (Olympus IX-71)
Quantitative gene expression (LC 1.5, LC480, Roche)
RNA interference
Genome-wide transcription (IScan, Illumina)
Chip-based Planar “patch-clamp” (Port-a-Patch, Nanion)
Flow cytometer (FACSCalibur, BD)
*(accessible at Ege Univ.)

5. Dept. of Internal Medicine E-mail: metiner.tosun@iue.edu.tr
Contact Details
Metiner Tosun, Ph.D.
Faculty of Medicine,
Dept. of Internal Medicine