1. Center of Excellence in Cancer Research (CECR)
Director, Dr. Mohamed L. Salem, PhD
Prof. of Immunology
Tanta University, Egypt

2. Tanta University Educational Hospital
Location
Location
Tanta University Educational Hospital

3. Established Date Established Date
June 2013 through a project funded by Science and Technology development Fund (STDF)
PI: Prof. Mohamed Labib Salem
Affiliation: Tanta University

4. Research Team Dr. Mohamed L.Salem Dr. Mohamed Ramadan Dr. Said Hammad
Prof. of immunology
Dr. Mohamed Ramadan
Prof. of Pediatric
Dr. Said Hammad
Prof. of clinical Pathology
Dr. Mohamed Attia
Assis. Prof. of Clinical Pathology
Dr. Abdel-Aziz Zidan
PhD of Immunology
Ms. Mona Fouad Zidan
Researcher Assistant

5. Research Team

6. Organogram

7. CECR Main Project
Correlating immunologic and clinical performance between genomic, transcriptomic and proteomic profiling of circulating tumor cells versus primary tumor cells

8. CECR Core Facility

9. Flow Cytometry Unit BD FACSCanto II Cytometer Nomination:
The BD FACSCanto derives its name from the musical term “bel canto” meaning operatic singing that focuses on ease and purity of tone with an agile and precise vocal technique.

10. Specifications of the instrument
The BD FACSCanto system has a capacity for three lasers, eight-parameter detection while capturing particle sizes from 0.5 to 50 μm in diameter.
Digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting
Multiparameter detection helps in:
Advanced cell subset analysis.
Multiparameter functional studies.
Soluble protein detection.
Quantification studies for cell-surface antigens and soluble proteins.
Decreases the total sample volume necessary for a specific project as more parameters.

11. Research & Clinical applications of the instrument
Specifications of the instrument
Research & Clinical applications of the instrument
Immunophenotyping by flow cytometry is an important tool in the diagnosis and staging of patients with a haematological neoplasm as well as solid tumors.
Some of the flow cytometry applications in diagnosis of different diseases are listed below:
Analysis of Leukemias and Lymphomas
Detection of minimal residual disease
Stem cell enumeration
Analysis of different types of solid tumors.
Detection of autoantibodies
BD FACSCanto Applications
Reticulocyte analysis
Detection of Immunodeficiency diseases
Detection of HIV infection

12. Cell Sorting Unit
The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high- quality , viable cells in a high density format , ready for use in the application of your choice
Benefits:
Access a wide range of samples.
Use your own capture criteria.
Capture intact, pure cells.
Minimize Sample Diluation
Save Time
Save Space
Utilize validated CTC analysis workflows.

13. Cell Sorting Unit IsoFlux Applications Enumeration Immunofluorescence
Fluorescence in situ hybridization
Next generation sequencing
Mutation detection
Gene expression

14. Genomic & Proteomic Unit
Microarray
A high throughput technology that allows detection of thousands of genes simultaneously.
Features:
High samples throughput.
Laser excitation at 532nm, dual- laser excitation .
Scanning pixel resolution 2µm
Scan sequence: simultaneous scan and independent scan.
Scan/read time: approximately 5 samples/hour.

15. Microarry Applications
Genomic & Proteomic Unit
Microarry Applications
Transiptome
mRNA Expression
miRNA Expression
Splice Variance
Genome Structure
DNA Copy number
Genome Variation
Single Nucleotide Polymorphism
Copy number Variations
Proteome
Protein-Protein Interactions
DNA – Protein inreraction
Epigenetics
DNA Methylation
Chromatin Structure

16. Genomic & Proteomic Unit
LumineX200

17. Genomic & Proteomic Unit
Flexible Multiplexing Technology
Open Platform for Genomic & Proteomic assays
Life Science Research & Diagnostic assays available.
Broad menu of commercial assays and laboratory developed tests
Multiplex up to 500 tests in one well of microliter plate.
Decrease sample volume requirements.
Reduce assay reagent volume, expense & labor.
Generate more information on interrelationships between analytes within a single sample.

18. Assay Development Tools
Genomic & Proteomic Unit
Luminex Applications
Clinical Diagnostics
Clinical Research
Life Science Research
Biosurveillance
Assay Development Tools

19. CECR Collaborators
CECR Collaborators

20. CECR Conferences Monday 24 February 2014 The 1st Annual Conference of
The Center of Excellence in Cancer Research
Next Generation Cancer Research & Diagnosis
In Collaboration with
Research Centers of Excellence
(STDF- Affiliated Centers)