1. The Analysis of Stomatal Density and Leaf Geometries in Plant Tissues
Visual and Image Processing
Rahman Tashakkori, Luke Rice, and Bahar Akhtar
Appalachian State University
The Analysis of Stomatal Density and Leaf Geometries in Plant Tissues
Karunesh Polireddy &
Akash Kejriwal

2. Objective Analyze the factors affecting stomatal density
Precipitation
Temperature
Visualize the geometric figures on leaves and identify any geometric similarities between plants of the same species
Previous work by environmentalists studying the atmosphere

3. Stomata Density - Method
The stomatal density was measured using a Proscope HR Microscope with a 400x magnification and 640x480 resolution.

4. Stoma seen at 400x magnification
Stomatal Density
Stomata –mouth-like structures that regulate the gases and liquid that enter and leave a leaf
Stoma seen at 400x magnification

5. Stomatal Density
Correlation w/ Temperature: As temperature increases, stomatal density decreases.

6. Stomatal Density
Correlation w/ Precipitation: As precipitation increases, stomatal density increases.

7. Leaf Geometry
Leaf Geometry – the geometrical shapes found on the plant epidermis.
Can be similar or can be different as well.
Geometries of Rhododendron and Hasta leaves were compared

8. Leaf Geometry – Method
Leaf geometries were found using “The Leafinator” created by Clint Guin.
The Leafinator, a program, measured average area, perimeter, hull area, and hull perimeter.

9. Leaf Geometry - Rhododendron
Three types were observed.
Type 1 and Type 2 were similar but Type 3 was not.
Leaf geometry is a varying factor. They can be different or similar between a species.

10. Leaf Geometry - Hasta Three types were observed.
Problem – lack of geometry
Resulting in skewed and uncorrelated data.

11. Conclusion – Stomatal Density
Can identify a plant’s species and biome.
The agave bovicornuta and poinsettia cactus have smaller density than daisies, squash, zucchinis, and shrubs.
This is due to environmental factors (temperature precipitation).

12. Conclusion – Leaf Geometry
Leaf Geometry shows relation between plants of same species
However, not all are similar.
Hasta leaves did not show any geometry on the epidermis.

13. References
[1] Broussard, Dillon, Tobi Ray, and Brittany Schaneman. "Stomata Mechanics: How a Stomata Works." Stomata Mechanics: How a Stomata Works. N.p., n.d. Web. 18 July < [2] Xu, Zhenzhu, and Guangsheng Zhou. "Responses of Leaf Stomatal Density to Water Status and Its Relationship with Photosynthesis in a Grass." PMC. US National Library of Medicine National Institutes of Health, 22 July Web. 17 July < [3] Meatyard, Barry, and Mary MacDonald. "Measuring Stomatal Density." Science & Plants for Schools. N.p., n.d. Web. 18 July < [4] "Weather History for Boone, North Carolina." Weather History for Boone, North Carolina. Yankee Publishing, 18 July Web. 18 July [5] "7-Day Zone Forecast for Mojave Desert Including the Mojave National Preserve." 7-Day Zone Forecast for Eastern Mojave Desert Including the Mojave National Preserve. N.p., n.d. Web. 18 July < [6] Knox, Gary W. "About Desert Plants." Desert Plants. N.p., n.d. Web. 18 July < [7] "Types of Photosynthesis." Types of Photosynthesis. Pima Community College, n.d. Web. 15 July [8] "Agave Bovicornuta at San Marcos Growers." Plant Information. N.p., n.d. Web. 19 July < [9] "The Geometry of Leaves." Education.com. N.p., n.d. Web. 22 July 2013.

14. Acknowledgements
Thanks to our professors for helping us carry out this project. Dr. Rahman Tashakkori Mr. Luke Rice Ms. Bahar Akhtar And others.. Dr. Carol Babyak Mr. Clint Guin