1. Screening of Anti-inflammatory Drugs
Lab # 4

2. Outline Describe the inflammatory response
Anti-inflammatory drugs; MOA, Uses, SE
Explain methods for screening of Anti-inflammatory drugs
Discuss the lab work
Gamra Hamad

3. Inflammation
Response of immune system to foreign organism or antigenic substance
Characterized by edema, erythema, swelling, and pain or tenderness
Gamra Hamad

4. Inflammatory Mediators
Phospholipids
Gamra Hamad

5. Phases of inflammation
Acute: vasodilation & increase capillary permeability
Delayed: infiltration of leukocytes and phagocyte cells
Chronic proliferative: tissue degeneration and fibrosis
Gamra Hamad

6. Anti-inflammatory Drugs
Steroidal: ex. Corticosteroids
(inh. gene transcription )
Non-steroidal Anti-inflammatory Drugs (NSAID)
(inh. COX)
Examples: ASA, indomethacin, ibuprofen
Selective COX-2 inhibitors
Gamra Hamad

7. NSAID Uses: rheumatoid arthritis
SE: GI adverse effects, renal insufficiency, bronchospasm
Gamra Hamad

8. Screening methods For Anti-inflammatory Drugs
Granuloma Pouch edema methods
Croton oil
Control vs. treated
Calculate % inhibition
Adjuvant-induced arthritis
ID Tubercle bacilli
Calculate % change in edema & body wt. over 14 days
Gamra Hamad

9. Screening methods For Anti-inflammatory Drugs
Rat-paw edema
Animal 6 rats (3 control, 3 treated)
Drugs: carragenin (2%, 0.1 ml SC.), indomethacin 2 mg/kg IP
Gamra Hamad

10. Cont. Control group Treated group Rt. Lt. 1 2.9 1.5 1.9 1.3 2 3.1 1.7
1.4 3
1.6
Mean A B C D
Gamra Hamad

11. Calculation % of edema produced by carragenin alone
= A – B X = X % B
% of edema produced by carragenin after ttt with indomethacin
= C – D X = Y % D
% reduction of edema by indomethcin
= X –Y= %
Gamra Hamad