1. CELLION Technical Report
Tilman Butz
Universität Leipzig
CELLION Midterm Meeting
Bordeaux
3.2.–

2. Map of Sites

3. Table of Contents Introduction
Components of NMPs with external beams Accelerators and ion sources Horizontal vs. vertical beamlines Beam formation Exit windows Ion detectors and online microscopy Petri dishes Target translation stages Hit accuracy Automatic cell recognition Track visualization
Summary

4. Cell to cell communication !
Introduction
Random irradiation
superposition of various interaction points => mixture of cellular responses
Targeted irradiation
selected interaction point
counted number of ions => specific cellular responses
Cell to cell communication !

5. Accelerators and Ion Sources

6. Linear Energy Transfer
A. Hauptner, S. Dietzel, G.A. Drexler, P. Reichart, R. Krücken, T. Cremer, A.A. Friedl, G. Dollinger,
Radiat. Environ. Biophys 42 (2004) 237.

7. 2 MV Tandetron at Guildford

8. intermediate image distance
A high demagnification system for the sub-micron region
5000
3150
100 40
250
object
image
Dimensions in mm.
Not to scale.
a0 =7.5
300
beam axis
cross-over X
intermediate image distance
working distance -A B C -D -C
A long working distance quadrupole doublet – triplet setup for 3 MeV protons allowing :
High demagnifications (65 X 100) keeping spherical aberrations at a reasonable level.
Intermediate beam focus and cross over for real imaging, diagnostics and fine tuning of the doublet
Comfortable intermediate (300 mm) and working distances (250 mm) in analysis or cellular irradiation configuration
A total beam length less than 10 metres
100 mm long OM 50 quadrupoles from Oxford Microbeams Ltd., U.K. (bore gap : 15 mm)
Centre d’Etudes Nucléaires de Bordeaux-Gradignan

9. Horizontal vs. vertical beam lines
Microbeam room
4MV Van de Graaff
Gray Cancer Institute, London

10. Horizontal vs. vertical beam lines
LIPSION, Leipzig

11. Horizontal vs. vertical Petri dishes
medium
residual
medium
for vertical beams
for horizontal beams

12. Beam Formation: Collimation

13. Beam Formation: Collimation
Legnaro

14. Beam Formation: Focusing
Scanner
magnetic lens system
object
slits
aperture
slits
beam
focus
Microprobe beam line at LIPSION, Leipzig

15. Exit Windows

16. Exit Windows
scanner
exit window

17. Proton Beam in Air

18. External Beam Line Properties

19. Ion Detectors and On-line Microscopy
CCD camera + intensifier
optical table
collimator assembly
stage
stage support & Z drive
light source
objective
support and alignment
GCI, London

20. Ion Detectors and On-line Microscopy
GCI, London

21. Single Ion Transmission Detector
GSI, Darmstadt
IFJ, Kraków
(Kraków plans to install a gas
proportional counter like RARAF) e-
secondary electrons from exit window allow single ion detection with nearly 100% efficiency
Channeltron

22. Petri Dish
GCI, London

23. Petri Dish
Petri dishes used
in Leipzig and Kraków
LIPSION, Leipzig

24. (polypropylene 4 µm + Cell-Tack)
Petri Dish
BACK
FRONT
1 cm
Polymer
culture support layer
(polypropylene 4 µm + Cell-Tack)
glass window
CENBG, Bordeaux

25. Translation Stage
fast translation stage using voice coils

26. Hit Accuracy Endothelial cell LIPSION, Leipzig
with nucleus of about 15 µm × 10 µm
LIPSION, Leipzig

27. Automatic Cell Recognition
CCD camera
cell finding & positioning
microscope
computer control
shutter
stage
detector
particle microbeam
GCI, London

28. Automatic Cell Recognition
Carbon 4.8MeV/u; 5 hits, 3µm distance
GSI, Darmstadt
Recognised cell nuclei marked by crosses.
Red cross: nucleus selected for irradiation

29. Automatic Cell Recognition Work in Kraków

30. Track Visualization: Foci
patterned
irradiation
10 µm
targeted
irradiation

31. Summary
Nuclear microprobes are state of the art tools to deliver a counted number of ions to cellular targets with micron and sub-micron precision.
All state of the art detection schemes for the cellular response have to be applied in order to study low dose biological effects in detail.
The rapid growth of the European nuclear microprobe community with radiobiological programs is encouraging and parallels worldwide efforts.